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Wavelength Specific Patterns of p53 Induction in Human Skin following Exposure to UV Radiation¹

Departments of Dermatology [C. C., A. G. Q., P. M. F., J. L. R.] and Pathology [B. A.], University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NEI 4LP, United Kingdom

We report that, in human skin, exposure to equally erythemogenic doses of UVA, UVB, and UVC increases immunocytochemically detected p53 in a wavelength-specific pattern. UVC produced immunostaining confined to the upper epidermis. With UVB, staining was seen throughout the epidermis, whereas with UVA staining predominated in the basal layer. The results with UVB and UVC are understandable on the basis of their known differences in penetration, whereas those with UVA are not. This suggests that within one cell type the pattern of p53 response to UV radiation is wavelength dependent.

¹ This work was supported by the North of England Cancer Research Campaign. C. C. is in receipt of a University of Newcastle Research Committee Ph.D. Studentship and A. G. Q is a Medical Research Council Training Fellow.

² To whom requests for reprints be addressed, at University Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4LP, United Kingdom.

Received 3/ 3/93. Accepted 5/ 4/93.

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