This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Haran-Ghera, N. Articles by Fan, H. Search for Related Content PubMed PubMed Citation Articles by Haran-Ghera, N. Articles by Fan, H.

Lymphomagenesis in AKR.Fv-1^b Congenic Mice¹

Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel [N. H-G., A. P.], and Department of Molecular Biology and Biochemistry, Cancer Research Institute, University of California, Irvine, California 92717 [B. K. B., H. F.]

In the AKR.Fv-1^b congenic strain the Fv-1ⁿ allele of the AKR/J mice was substituted with the Fv-1^b allele, thereby limiting viral replication and spread of the endogenous N-tropic murine leukemia virus. As a result of this genetic change AKR.Fv-1^b mice develop a low spontaneous incidence (7%) of T-cell lymphomas and about 28% of Ly-1⁺ B-cell lymphomas are observed in old mice. Characteristic changes in thymus subpopulations of AKR/J mice (related to the formation of the dual tropic mink cell focus inducing (MCF) type virus in the thymus) were not observed in the thymus of AKR.Fv-1^b mice. In contrast to the low susceptibility to spontaneous T-cell lymphoma development, these mice were highly sensitive to fractionated irradiation or to radiation leukemia virus (a mixture of N- and B-tropic viruses) induced T-cell lymphoma. Potential lymphoma cells (that would ultimately develop into Ly-1⁺ B-cell lymphomas) were demonstrated in bone marrow and spleens of 16–24-month-old mice. Analysis of the Ly-1⁺ IgM⁺ B-cell population in spleens of 18-month-old mice revealed a significant increase in this population (35% versus 2% in young spleens). The spontaneous Ly-1⁺ B-cell lymphoma incidence could be enhanced (up to 77%) by in vivo administration of anti-CD8 monoclonal antibody or IL-4 to 18-month-old mice.

Virological analysis of T/B-cell lymphomas for class I MCF viruses indicated that Class I MCF development was tightly correlated with T-lymphoma development (except radiation induced tumors that showed no MCF provirus involvement). In contrast, Ly-1⁺ B-cell lymphoma development was independent of Class I MCF pathogenic virus involvement.

¹ This research was supported by Grant 147/91 to N. H. G. from the Israel Science Foundation.

Received 12/21/92. Accepted 5/ 6/93.

This article has been cited by other articles:

				J. Lovmand, A. B. Sorensen, J. Schmidt, M. Ostergaard, A. Luz, and F. S. Pedersen B-Cell Lymphoma Induction by Akv Murine Leukemia Viruses Harboring One or Both Copies of the Tandem Repeat in the U3 Enhancer J. Virol., July 1, 1998; 72(7): 5745 - 5756. [Abstract] [Full Text] [PDF]