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Haematology-Oncology Department [J. E. H., D. K., B. F., J-X. M., R. E. S., A. D.] and Gastroenterology Unit [R. N. B.], The Queen Elizabeth Hospital, Woodville, South Australia, 5011, Australia
The presence of tumor cells in the circulation may predict disease recurrence and metastases. We have developed a sensitive technique for the detection of carcinoma cells in blood, using immunomagnetic beads to enrich for epithelial cells and the polymerase chain reaction to identify a tumor marker. The colon carcinoma cell line SW480, homozygous for a K-ras codon 12 mutation, was used to establish optimal conditions. The SW480 cells were serially diluted in normal blood and incubated with immunomagnetic beads labeled with a monoclonal antibody specific for epithelial cells. Cells bound to the beads were retrieved using a magnetic field and the presence of K-ras codon 12 mutations determined by a polymerase chain reaction based analysis. SW480 cells could be detected in dilutions up to 1 SW480 cell/105 leukocytes in whole blood.
1 To whom requests for reprints should be addressed.
Received 3/22/93. Accepted 6/16/93.
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