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Interleukin 10 Transfected into Chinese Hamster Ovary Cells Prevents Tumor Growth and Macrophage Infiltration¹

Institute of Immunology, Klinikum Steglitz, The Free University of Berlin, Hindenburgdamm 27, 12203 Berlin. [G. R., S. K-K., G. H., T. D., T. B.] and Institute of Immunology, University of Mainz, Mainz [C. H., E. S.], Germany

Expression of cytokines in tumor cells provides a sensitive modality to analyze the consequences of local cytokines in vivo on tumor infiltrating cells and tumorigenicity. We have transfected Chinese hamster ovary (CHO) cells with an interleukin 10 (IL-10) expression vector. CHO-IL10 cells although unaltered with respect to their in vitro growth lost tumorigenicity, both in nude and in SCID mice and in an IL-10 dose dependent manner. In addition, CHO-IL10 cells suppressed the growth of equal numbers of coinjected but not of contralaterally injected CHO cells. Immunohistology with anti-CR3/Mac-1 and anti-Mac-3 monoclonal antibodies revealed that CHO tumors were substantially infiltrated by macrophages. However, in CHO-IL10 tumors macrophages were virtually absent within the tumor tissue. Our results suggest that IL-10 indirectly suppresses tumor growth of certain tumors by inhibiting infiltration of macrophages which may provide tumor growth promoting activity.

¹ This work was supported by a grant from the Deutsche Krebshilfe, Mildred Scheel-Stiftung, e.V.

² To whom requests for reprints should be addressed. Present address: New York Hospital-Cornell Medical Center, Department of Medicine, Division of Allergy-Immunology, 525 E. 68th Street, New York, NY 10021.

Received 6/28/93. Accepted 8/ 4/93.

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