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Cyclin A Expression in Human Hematological Malignancies: A New Marker of Cell Proliferation¹

U.370, CHU Necker, 156 rue de Vaugirard, 75015 Paris [P. P., F. Z., M. S., C. B.], Service d'Hematologic, Hôpital Hotel Dieu, Paris [A. M. S., J. P. M.],, Service d'Hematologie, Hôpital Cochin, Paris [J. M., F. D.], and Service d'Hepatologie, Hôpital Laennec, Paris [C. B.], France

² To whom requests for reprints should be addressed, at Unite Inserm 75, CHU Necker, 156 rue de Vaugirard, 75015 Paris, France.

Several in vitro studies have shown that the cyclin A gene is expressed and plays an important role in both the S and G₂-M phases of the cell cycle. We analyzed cells from the blood and bone marrow of patients with various, mostly neoplastic, hematological disorders to determine whether (a) the cyclin A protein level correlated with that of cyclin A RNA and (b) cell distribution among the different phases of the cell cycle correlated with cyclin A RNA expression. Thirty-eight patients were studied by means of dot blot and Western blot techniques for cyclin A RNA and protein accumulation, and 21 were also studied for cell cycle distribution by using flow cytometric analysis. Semiquantitative studies were based on densitometric computerized evaluation of dot and Western blots. There was a very strong positive correlation between cyclin A RNA and protein expression (r = 0.99; P < 0.00005), indicating that cyclin A accumulation is regulated in these cells at a transcriptional level. There was also a highly significant positive correlation between cyclin A RNA expression and the cumulative percentage of cells in S plus G₂-M phase (r = 0.98; P < 0.00005). Therefore, this in vivo study shows that the expression of cyclin A RNA and protein in human hematological malignancies correlates with the percentage of cells in S plus G2-M phase and identifies cyclin A as a new potential cell proliferation index in oncology.

¹ Work supported by ARC, LNC, Fondation de France, INSERM, CEE.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 10/21/92. Accepted 11/24/92.

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