Cancer Research The Future of Cancer Research: Science and Patient Impact Cancer Health Disparities Conference 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
[Cancer Research 53, 354-361, January 15, 1993]
© 1993 American Association for Cancer Research
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takada, A.
Right arrow Articles by Kannagi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takada, A.
Right arrow Articles by Kannagi, R.

Contribution of Carbohydrate Antigens Sialyl Lewis A and Sialyl Lewis X to Adhesion of Human Cancer Cells to Vascular Endothelium1

Akiko Takada, Katsuyuki Ohmori, Tomoya Yoneda, Kiyotaka Tsuyuoka, Akira Hasegawa, Makoto Kiso and Reiji Kannagi2

Laboratory of Experimental Pathology, Research Institute, Aichi Cancer Center, Nagoya, 464 [A. K., K. T., R. K.]; Department of Laboratory Medicine, Kyoto University, School of Medicine, Kyoto, 606 [K. O., T. Y]; and Department of Applied Bio-organic Chemistry, Gifu University, Faculty of Agriculture, Gifu, 501-11 [A. H., M. K.], Japan

2 To whom requests for reprints should be addressed, at Laboratory of Experimental Pathology, Research Institute, Aichi Cancer Center, 1-1 Kanokoden, Chikusaku, Nagoya, 464, Japan.

The carbohydrate antigen, sialyl Lex, is known to be a ligand for the cell adhesion molecule called ELAM-1 (E-selectin, endothelial cell leukocyte adhesion molecule-1), which is present on cytokine-activated human endothelial cells. Recently, we reported that another carbohydrate antigen, sialyl Lea, can also serve as a ligand for ELAM-1 (A. Takada, K. Ohmori, N. Takahashi, K. Tsuyuoka, K. Yago, K. Zenita, A. Hasegawa, and R. Kannagi, Biochem. Biophys. Res. Commun., 179: 713–719, 1991). Both sialyl Lex and sialyl Lea are expressed in many human malignant cells. In order to assess the contribution of these carbohydrate antigens to the adhesion of human malignant cells to vascular endothelium, we selected a panel of 12 cultured human epithelial cancer cell lines and a panel of 12 human leukemia cell lines which express sialyl Lex and/or sialyl Lea antigens. All 12 epithelial cancer cell lines exhibited a clearly ELAM-1 -dependent adhesion to cytokine-activated human umbilical vein endothelial cells, while only 3 of the 12 leukemia cell lines exhibited significant participation of ELAM-1 in the adhesion. With regard to epithelial cancer cells, the adhesion of 6 cancer cell lines, mostly of colon and pancreas origin, was dependent almost exclusively on sialyl Lea. A significant contribution of the sialyl Lex antigen was noted in the adhesion of the other 6 cell lines, including cancers of lung and liver origin. These results imply that the sialyl Lea/ELAM-1 adhesion system, as well as the sialyl Lex/ELAM-1 adhesion system, plays an important role in the adhesion of human cancer cells to human umbilical vein endothelial cells. With regard to leukemia cells, on the other hand, adhesion of the 3 leukemia cell lines that showed ELAM-1-dependent adhesion was mediated by the sialyl Lex antigen, and none of these leukemia cell lines expressed sialyl Lea or exhibited sialyl Lea-dependent adhesion.

1 This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture, Japan (03557114 and 03258218).

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 6/29/92. Accepted 10/28/92.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Liang and C. Dong
Integrin VLA-4 enhances sialyl-Lewisx/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C701 - C707.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Kimura, K. Ohmori, K. Miyazaki, M. Izawa, Y. Matsuzaki, Y. Yasuda, H. Takematsu, Y. Kozutsumi, A. Moriyama, and R. Kannagi
Human B-lymphocytes Express {alpha}2-6-Sialylated 6-Sulfo-N-acetyllactosamine Serving as a Preferred Ligand for CD22/Siglec-2
J. Biol. Chem., November 2, 2007; 282(44): 32200 - 32207.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
N. Malagolini, D. Santini, M. Chiricolo, and F. Dall'Olio
Biosynthesis and expression of the Sda and sialyl Lewis x antigens in normal and cancer colon
Glycobiology, July 1, 2007; 17(7): 688 - 697.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Eguchi, Y. Ikeda, T. Ookawara, S. Koyota, N. Fujiwara, K. Honke, P. G. Wang, N. Taniguchi, and K. Suzuki
Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x-mediated cell adhesion
Glycobiology, November 1, 2005; 15(11): 1094 - 1101.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. I. Kawamura, R. Kawashima, R. Fukunaga, K. Hirai, N. Toyama-Sorimachi, M. Tokuhara, T. Shimizu, and T. Dohi
Introduction of Sda Carbohydrate Antigen in Gastrointestinal Cancer Cells Eliminates Selectin Ligands and Inhibits Metastasis
Cancer Res., July 15, 2005; 65(14): 6220 - 6227.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. D. Belanger and Y. St-Pierre
Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma
Blood, June 15, 2005; 105(12): 4800 - 4806.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. Kikuchi, H. Shinohara, C. Nonomura, H. Ando, S. Takaku, H. Nojiri, and M. Nakamura
Not core 2 {beta}1,6-N-acetylglucosaminyltransferase-2 or -3 but -1 regulates sialyl-Lewis x expression in human precursor B cells
Glycobiology, March 1, 2005; 15(3): 271 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. V. Beum, H. Basma, D. R. Bastola, and P.-W. Cheng
Mucin biosynthesis: upregulation of core 2 {beta}1,6 N-acetylglucosaminyltransferase by retinoic acid and Th2 cytokines in a human airway epithelial cell line
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L116 - L124.
[Abstract] [Full Text] [PDF]

Home page
 Jpn J Clin OncolHome page
T. Matsui, H. Kojima, H. Suzuki, H. Hamajima, H. Nakazato, K. Ito, A. Nakao, and J. Sakamoto
Sialyl Lewisa Expression as a Predictor of the Prognosis of Colon Carcinoma Patients in a Prospective Randomized Clinical Trial
Jpn. J. Clin. Oncol., October 1, 2004; 34(10): 588 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Miyazaki, K. Ohmori, M. Izawa, T. Koike, K. Kumamoto, K. Furukawa, T. Ando, M. Kiso, T. Yamaji, Y. Hashimoto, et al.
Loss of Disialyl Lewisa, the Ligand for Lymphocyte Inhibitory Receptor Sialic Acid-Binding Immunoglobulin-Like Lectin-7 (Siglec-7) Associated with Increased Sialyl Lewisa Expression on Human Colon Cancers
Cancer Res., July 1, 2004; 64(13): 4498 - 4505.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Koike, N. Kimura, K. Miyazaki, T. Yabuta, K. Kumamoto, S. Takenoshita, J. Chen, M. Kobayashi, M. Hosokawa, A. Taniguchi, et al.
Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates
PNAS, May 25, 2004; 101(21): 8132 - 8137.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Kaufmann, S. Black, and B. Huppertz
Endovascular Trophoblast Invasion: Implications for the Pathogenesis of Intrauterine Growth Retardation and Preeclampsia
Biol Reprod, July 1, 2003; 69(1): 1 - 7.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Tsuchida, T. Okajima, K. Furukawa, T. Ando, H. Ishida, A. Yoshida, Y. Nakamura, R. Kannagi, M. Kiso, and K. Furukawa
Synthesis of Disialyl Lewis a (Lea) Structure in Colon Cancer Cell Lines by a Sialyltransferase, ST6GalNAc VI, Responsible for the Synthesis of {alpha}-Series Gangliosides
J. Biol. Chem., June 13, 2003; 278(25): 22787 - 22794.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Tei, N. Kawakami-Kimura, O. Taguchi, K. Kumamoto, S. Higashiyama, N. Taniguchi, K.'i. Toda, R. Kawata, Y. Hisa, and R. Kannagi
Roles of Cell Adhesion Molecules in Tumor Angiogenesis Induced by Cotransplantation of Cancer and Endothelial Cells to Nude Rats
Cancer Res., November 1, 2002; 62(21): 6289 - 6296.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Ohyama, S. Kanto, K. Kato, O. Nakano, Y. Arai, T. Kato, S. Chen, M. N. Fukuda, and M. Fukuda
Natural killer cells attack tumor cells expressing high levels of sialyl Lewis x oligosaccharides
PNAS, October 15, 2002; 99(21): 13789 - 13794.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kanamori, N. Kojima, K. Uchimura, T. Muramatsu, T. Tamatani, M. C. Berndt, G. S. Kansas, and R. Kannagi
Distinct Sulfation Requirements of Selectins Disclosed Using Cells That Support Rolling Mediated by All Three Selectins under Shear Flow. L-SELECTIN PREFERS CARBOHYDRATE 6-SULFATION TO TYROSINE SULFATION, WHEREAS P-SELECTIN DOES NOT
J. Biol. Chem., August 30, 2002; 277(36): 32578 - 32586.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Zhang, J. Nakayama, C. Ohyama, M. Suzuki, A. Suzuki, M. Fukuda, and M. N. Fukuda
Sialyl Lewis X-dependent Lung Colonization of B16 Melanoma Cells through a Selectin-like Endothelial Receptor Distinct from E- or P-Selectin
Cancer Res., August 1, 2002; 62(15): 4194 - 4198.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A.-M. Khatib, G. Siegfried, M. Chretien, P. Metrakos, and N. G. Seidah
Proprotein Convertases in Tumor Progression and Malignancy : Novel Targets in Cancer Therapy
Am. J. Pathol., June 1, 2002; 160(6): 1921 - 1935.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Borsig, R. Wong, R. O. Hynes, N. M. Varki, and A. Varki
Synergistic effects of L- and P-selectin in facilitating tumor metastasis can involve non-mucin ligands and implicate leukocytes as enhancers of metastasis
PNAS, February 19, 2002; 99(4): 2193 - 2198.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Kumamoto, Y. Goto, K. Sekikawa, S. Takenoshita, N. Ishida, M. Kawakita, and R. Kannagi
Increased Expression of UDP-Galactose Transporter Messenger RNA in Human Colon Cancer Tissues and Its Implication in Synthesis of Thomsen-Friedenreich Antigen and Sialyl Lewis A/X Determinants
Cancer Res., June 1, 2001; 61(11): 4620 - 4627.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Borsig, R. Wong, J. Feramisco, D. R. Nadeau, N. M. Varki, and A. Varki
Heparin and cancer revisited: Mechanistic connections involving platelets, P-selectin, carcinoma mucins, and tumor metastasis
PNAS, March 13, 2001; 98(6): 3352 - 3357.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Minami, J. Furui, and T. Kanematsu
Role of Carcinoembryonic Antigen in the Progression of Colon Cancer Cells That Express Carbohydrate Antigen
Cancer Res., March 1, 2001; 61(6): 2732 - 2735.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
K.-i. Kobayashi, S. Matsumoto, T. Morishima, T. Kawabe, and T. Okamoto
Cimetidine Inhibits Cancer Cell Adhesion to Endothelial Cells and Prevents Metastasis by Blocking E-selectin Expression
Cancer Res., July 1, 2000; 60(14): 3978 - 3984.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
K.-i. Kozaki, O. Miyaishi, T. Tsukamoto, Y. Tatematsu, T. Hida, T. Takahashi, and T. Takahashi
Establishment and Characterization of a Human Lung Cancer Cell Line NCI-H460-LNM35 with Consistent Lymphogenous Metastasis via Both Subcutaneous and Orthotopic Propagation
Cancer Res., May 1, 2000; 60(9): 2535 - 2540.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. Izawa, K. Kumamoto, C. Mitsuoka, A. Kanamori, K. Ohmori, H. Ishida, S. Nakamura, K. Kurata-Miura, K. Sasaki, T. Nishi, et al.
Expression of Sialyl 6-Sulfo Lewis X Is Inversely Correlated with Conventional Sialyl Lewis X Expression in Human Colorectal Cancer
Cancer Res., March 1, 2000; 60(5): 1410 - 1416.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. Aubert, L. Panicot, C. Crotte, P. Gibier, D. Lombardo, M.-O. Sadoulet, and E. Mas
Restoration of {{alpha}}(1,2) Fucosyltransferase Activity Decreases Adhesive and Metastatic Properties of Human Pancreatic Cancer Cells
Cancer Res., March 1, 2000; 60(5): 1449 - 1456.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. N. Fukuda, C. Ohyama, K. Lowitz, O. Matsuo, R. Pasqualini, E. Ruoslahti, and M. Fukuda
A Peptide Mimic of E-Selectin Ligand Inhibits Sialyl Lewis X-dependent Lung Colonization of Tumor Cells
Cancer Res., January 1, 2000; 60(2): 450 - 456.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
T. Fujisaki, Y. Tanaka, K. Fujii, S. Mine, K. Saito, S. Yamada, U. Yamashita, T. Irimura, and S. Eto
CD44 Stimulation Induces Integrin-mediated Adhesion of Colon Cancer Cell Lines to Endothelial Cells by Up-Regulation of Integrins and c-Met and Activation of Integrins
Cancer Res., September 1, 1999; 59(17): 4427 - 4434.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. V. Beum, J. Singh, M. Burdick, M. A. Hollingsworth, and P.-W. Cheng
Expression of Core 2 beta -1,6-N-Acetylglucosaminyltransferase in a Human Pancreatic Cancer Cell Line Results in Altered Expression of MUC1 Tumor-associated Epitopes
J. Biol. Chem., August 27, 1999; 274(35): 24641 - 24648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. J. Kim, L. Borsig, H.-L. Han, N. M. Varki, and A. Varki
Distinct Selectin Ligands on Colon Carcinoma Mucins Can Mediate Pathological Interactions among Platelets, Leukocytes, and Endothelium
Am. J. Pathol., August 1, 1999; 155(2): 461 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
B. W. Weston, K. M. Hiller, J. P. Mayben, G. A. Manousos, K. M. Bendt, R. Liu, and J. C. Cusack Jr.
Expression of Human {{alpha}}(1,3)Fucosyltransferase Antisense Sequences Inhibits Selectin-mediated Adhesion and Liver Metastasis of Colon Carcinoma Cells
Cancer Res., May 1, 1999; 59(9): 2127 - 2135.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Isshiki, A. Togayachi, T. Kudo, S. Nishihara, M. Watanabe, T. Kubota, M. Kitajima, N. Shiraishi, K. Sasaki, T. Andoh, et al.
Cloning, Expression, and Characterization of a Novel UDP-galactose:beta -N-Acetylglucosamine beta 1,3-Galactosyltransferase (beta 3Gal-T5) Responsible for Synthesis of Type 1 Chain in Colorectal and Pancreatic Epithelia and Tumor Cells Derived Therefrom
J. Biol. Chem., April 30, 1999; 274(18): 12499 - 12507.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Kimura, C. Mitsuoka, A. Kanamori, N. Hiraiwa, K. Uchimura, T. Muramatsu, T. Tamatani, G. S. Kansas, and R. Kannagi
Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by cotransfection of alpha 1right-arrow3 fucosyltransferase VII and newly cloned GlcNAcbeta :6-sulfotransferase cDNA
PNAS, April 13, 1999; 96(8): 4530 - 4535.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Elble, J. Widom, A. D. Gruber, M. Abdel-Ghany, R. Levine, A. Goodwin, H.-C. Cheng, and B. U. Pauli
Cloning and Characterization of Lung-Endothelial Cell Adhesion Molecule-1 Suggest It Is an Endothelial Chloride Channel
J. Biol. Chem., October 31, 1997; 272(44): 27853 - 27861.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Burdick, A. Harris, C. J. Reid, T. Iwamura, and M. A. Hollingsworth
Oligosaccharides Expressed on MUC1 Produced by Pancreatic and Colon Tumor Cell Lines
J. Biol. Chem., September 26, 1997; 272(39): 24198 - 24202.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Gallart, D. Roelcke, M. Blay, A. Pereira, A. Martinez, O. Masso, O. Vinas, M. Cid, J. Esparza, R. Molina, et al.
Anti-Sia-lb (Anti-Gd) Cold Agglutinins Bind the Domain NeuNAcalpha 2-3Gal in Sialyl Lewisx, Sialyl Lewisa, and Related Carbohydrates on Nucleated Cells and in Soluble Cancer-Associated Mucins
Blood, August 15, 1997; 90(4): 1576 - 1587.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Hiraiwa, T. Dohi, N. Kawakami-Kimura, M. Yumen, K. Ohmori, M. Maeda, and R. Kannagi
Suppression of Sialyl Lewis X Expression and E-selectin-mediated Cell Adhesion in Cultured Human Lymphoid Cells by Transfection of Antisense cDNA of an alpha 1right-arrow 3 Fucosyltransferase (Fuc-T VII)
J. Biol. Chem., December 6, 1996; 271(49): 31556 - 31561.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
J.-i. Ogawa, A. Sano, H. Inoue, and S. Koide
Expression of Lewis-Related Antigen and Prognosis in Stage I Non-Small Cell Lung Cancer
Ann. Thorac. Surg., February 1, 1995; 59(2): 412 - 415.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S.-H. Kim, M. Goto, and T. Akaike
Specific Binding of Glucose-derivatized Polymers to the Asialoglycoprotein Receptor of Mouse Primary Hepatocytes
J. Biol. Chem., September 14, 2001; 276(38): 35312 - 35319.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1993 by the American Association for Cancer Research.