This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Preston, S. R. Articles by Primrose, J. N. Search for Related Content PubMed PubMed Citation Articles by Preston, S. R. Articles by Primrose, J. N.

High Affinity Binding Sites for Gastrin Releasing Peptide on Human Gastric Cancer and Ménétrier's Mucosa¹

Academic Unit of Surgery [S. R. P., L. F. W., S. J-B., J. N. P.] and Department of Pathology [J. I. W.], St. James's University Hospital, Leeds, LS9 7TF, United Kingdom

The bombesin-like peptides gastrin releasing peptide (GRP) and neuromedin B are found in the submucosal and myenteric plexuses of the human gastrointestinal tract. These peptides are potent mitogens to Swiss 3T3 fibroblasts and are important autocrine growth factors in human small cell lung cancer cells. We have recently described the presence of receptors for the bombesin-like peptide, GRP, on the human gastric cancer cell line St42. In this study, we examined fresh resected gastric cancer and uninvolved mucosa from 23 patients for the presence of binding sites to the bombesin-like peptides. Thirteen of 23 gastric cancers expressed high affinity binding sites for bombesin (mean K_d = 3.42 nM; mean B_max = 40.5 pmol/mg protein), of which 12 were subsequently characterized and found to be of the GRP-preferring subtype. One of 23 mucosal samples specifically bound bombesin and was the only sample from a patient with Ménétrier's disease, a disorder of mucosal growth known to be premalignant. The early gastric cancer from this patient also possessed high affinity binding sites for GRP. This is the first description of binding sites to bombesin-like peptides on human gastric cancer and Ménétrier's mucosa. The role of bombesin/GRP antagonists in the treatment of gastric cancer warrants investigation.

¹ Supported by Grant L232 from the Yorkshire Cancer Research Campaign, Harrogate, United Kingdom.

² Funded by Glaxo Group Research, Ltd., Greenford, United Kingdom.

³ To whom requests for reprints should be addressed.

⁴ Funded by the Yorkshire Cancer Research Campaign.

Received 8/ 9/93. Accepted 9/17/93.

This article has been cited by other articles:

				D. Cornelio, R Roesler, and G Schwartsmann Gastrin-releasing peptide receptor as a molecular target in experimental anticancer therapy Ann. Onc., September 1, 2007; 18(9): 1457 - 1466. [Abstract] [Full Text] [PDF]

				T. W. Moody, L.-C. Sun, S. A. Mantey, T. Pradhan, L. V. Mackey, N. Gonzales, J. A. Fuselier, D. H. Coy, and R. T. Jensen In Vitro and in Vivo Antitumor Effects of Cytotoxic Camptothecin-Bombesin Conjugates Are Mediated by Specific Interaction with Cellular Bombesin Receptors J. Pharmacol. Exp. Ther., September 1, 2006; 318(3): 1265 - 1272. [Abstract] [Full Text] [PDF]

				B. Van Den Bossche and C. Van de Wiele Receptor Imaging in Oncology by Means of Nuclear Medicine: Current Status J. Clin. Oncol., September 1, 2004; 22(17): 3593 - 3607. [Abstract] [Full Text] [PDF]

				T. W. Moody, S. A. Mantey, T. K. Pradhan, M. Schumann, T. Nakagawa, A. Martinez, J. Fuselier, D. H. Coy, and R. T. Jensen Development of High Affinity Camptothecin-Bombesin Conjugates That Have Targeted Cytotoxicity for Bombesin Receptor-containing Tumor Cells J. Biol. Chem., May 28, 2004; 279(22): 23580 - 23589. [Abstract] [Full Text] [PDF]

				N Scott, E Millward, E J Cartwright, S R Preston, and P L Coletta Gastrin releasing peptide and gastrin releasing peptide receptor expression in gastrointestinal carcinoid tumours J. Clin. Pathol., February 1, 2004; 57(2): 189 - 192. [Abstract] [Full Text] [PDF]

				R. P. Thomas, M. R. Hellmich, C. M. Townsend Jr., and B. M. Evers Role of Gastrointestinal Hormones in the Proliferation of Normal and Neoplastic Tissues Endocr. Rev., October 1, 2003; 24(5): 571 - 599. [Abstract] [Full Text] [PDF]

				J. C. Reubi Peptide Receptors as Molecular Targets for Cancer Diagnosis and Therapy Endocr. Rev., August 1, 2003; 24(4): 389 - 427. [Abstract] [Full Text] [PDF]

				J.-C. Saurin, M. Fallavier, B. Sordat, J.-C. Gevrey, J.-A. Chayvialle, and J. Abello Bombesin Stimulates Invasion and Migration of Isreco1 Colon Carcinoma Cells in a Rho-dependent Manner Cancer Res., August 15, 2002; 62(16): 4829 - 4835. [Abstract] [Full Text] [PDF]

				H. J. Kim, B. M. Evers, D. A. Litvak, M. R. Hellmich, and C. M. Townsend Jr. Signaling mechanisms regulating bombesin-mediated AP-1 gene induction in the human gastric cancer SIIA Am J Physiol Cell Physiol, August 1, 2000; 279(2): C326 - C334. [Abstract] [Full Text] [PDF]