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Systematic HRAS Amplification in Ovary-independent Mammary Tumors: Correlation with Progressively Anaplastic Phenotypes¹

The University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville, Texas 78957

Progression of well differentiated rat mammary adenocarcinomas to very anaplastic phenotypes was found to correlate with a systematic and significant amplification of a mutant HRAS allele. Tumors with high amplification levels of this oncogene were analyzed by chromosomal in situ hybridization; in four of the cases the amplified sequences did not reside at the native chromosome 1 locus but were localized in a novel marker chromosome. The model described has potential as a reproducible system for the study of the chromosomal and cellular mechanisms operative "in vivo" for oncogene amplification.

¹ This study was supported by NIH Grant CA 48922.

² To whom requests for reprints should be addressed.

Received 6/18/93. Accepted 10/ 4/93.