This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cheng, X. Articles by Iliakis, G. Search for Related Content PubMed PubMed Citation Articles by Cheng, X. Articles by Iliakis, G.

Mitosis-promoting Factor Activity of Inducer Mitotic Cells May Affect Radiation Yield of Interphase Chromosome Breaks in the Premature Chromosome Condensation Assay¹

Thomas Jefferson University, Department of Radiation Oncology and Nuclear Medicine, Philadelphia, Pennsylvania 19107 [X.C., G.E.P., R.O., N.C., G.I.], and National Center for Scientific Research, Demokritos, Athens 153.10, Greece [G.E.P.]

³ To whom requests for reprints should be addressed, at Thomas Jefferson University, Department of Radiation Oncology and Nuclear Medicine, Thompson Building, Room B13, Philadelphia, PA 19107.

We measured mitosis-promoting factor (MPF) activity in two cell lines, CHO and HeLa, extensively used at mitosis as inducers in the assay of premature chromosome condensation to study the yield and the repair kinetics of radiation damage in interphase chromosomes of diverse cell lines. We found a 2.5-fold higher MPF activity in HeLa as compared to CHO mitotic cells per mg of crude extract protein. HeLa mitotic cells, when used as inducers of premature chromosome condensation, uncovered two times more interphase chromosome breaks in irradiated, nonstimulated human lymphocytes as compared to CHO mitotic cells. A 2-fold increase in the yield of interphase chromosome breaks with HeLa mitotics was also observed in G₁ cells from plateau-phase CHO cultures. Thus, MPF activity may be a contributing factor of the process that transforms radiation-induced DNA damage to chromosome breaks, and subsequently to other types of lethal chromosome aberrations. We speculate that the level and the control in the cell cycle of MPF activity may influence the radiosensitivity of cells to killing. The results strongly suggest that a direct comparison between the yields of interphase chromosome breaks measured in different laboratories may not be possible unless similar inducer cells with similar MPF activity are used.

¹ This work was supported by National Cancer Institute Grant IRO1 CA42026 awarded from NIH, Department of Health and Human Services.

² Supported in part by Grant F13P-CT92-0017 awarded by the Commission of the European Communities, Radiation Protection Programme.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 9/ 8/93. Accepted 10/19/93.

This article has been cited by other articles:

				V. I. Hatzi, G. I. Terzoudi, V. Makropoulos, C. Maravelias, and G. E. Pantelias Pre-irradiation exposure of peripheral blood lymphocytes to glutaraldehyde induces radiosensitization by increasing the initial yield of radiation-induced chromosomal aberrations Mutagenesis, March 1, 2008; 23(2): 101 - 109. [Abstract] [Full Text] [PDF]

				G. I. Terzoudi and G. E. Pantelias Cytogenetic methods for biodosimetry and risk individualisation after exposure to ionising radiation Radiat Prot Dosimetry, December 1, 2006; 122(1-4): 513 - 520. [Abstract] [Full Text] [PDF]

				G. I. Terzoudi, K. N. Manola, G. E. Pantelias, and G. Iliakis Checkpoint Abrogation in G2 Compromises Repair of Chromosomal Breaks in Ataxia Telangiectasia Cells Cancer Res., December 15, 2005; 65(24): 11292 - 11296. [Abstract] [Full Text] [PDF]

				P. Mosesso, E. Fonti, L. Bassi, C. L. Garcia, and F. Palitti The involvement of chromatin condensation in camptothecin-induced chromosome breaks in G0 human lymphocytes Mutagenesis, January 1, 1999; 14(1): 103 - 105. [Abstract] [Full Text] [PDF]