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Up-Regulation of Vascular Endothelial Growth Factor and Its Cognate Receptors in a Rat Glioma Model of Tumor Angiogenesis¹

Max-Planck-Institut für Psychiatrie, Abteilung Neurochemie [K. H. P, G. B., W. R.], and Max-Planck-Institut für Biochemie, Abteilung Molekularbiologie [B. M., A. U.], Am Klopferspitz 18a, 82143 Martinsried, Germany

² Present address: Klinikum der Philipps-Universität, Abteilung Neuropathologie, Baldingerstrasse 1, 35043 Marburg, Germany. To whom requests for reprints should be addressed.

We have recently shown that vascular endothelial growth factor (VEGF) is produced by human malignant glioma cells and acts on tumor endothelial cells, which express VEGF receptors, suggesting that VEGF is a regulator of tumor angiogenesis. To investigate the feasibility of antiangiogenic brain tumor therapy, we developed an intracerebral (i.c.) rat glioma model. We used two transplantable rat glioma cells lines, C6 and GS-9L, to analyze VEGF regulation in vitro and expression of VEGF and its high affinity tyrosine kinase receptors, flt-1 and flk-1, in vivo. Glioma cells were transplanted i.c. or s.c. into syngeneic rats. C6 gliomas exhibit morphological characteristics of human glioblastoma multiforme such as necroses with palisading cells. Immunocytochemistry with von Willebrand factor showed that C6 gliomas are highly vascularized and therefore show another prominent feature of human glioblastoma. GS-9L gliosarcomas were less vascularized. In situ hybridization showed that VEGF is expressed in vivo in rat glioma cells which reside along necrotic areas and therefore closely mimicks the expression pattern of VEGF observed in human glioblastoma. flt-1 and flk-1 are specifically expressed in endothelial cells in the tumor and at the border between tumor and normal brain but are absent from endothelial cells in the normal brain proper. The action of VEGF may therefore be restricted to tumor endothelium. Upregulation of VEGF, but not acid fibroblast growth factor, basic fibroblast growth factor, and platelet-derived growth factor B messenger RNA was observed in hypoxic C6 and GS-9L cells in vitro. These observations are consistent with a role for VEGF in tumor- and hypoxia-induced angiogenesis. Since the expression pattern of VEGF and its receptors in rat glioma appears to be indistinguishable from human glioblastoma multiforme, this model provides an excellent tool to study anti-angiogenic therapy.

¹ This study was supported by a grant from Deutsche Krebshilfe (Mildred-Scheel-Stiftung).

³ Present address: Max-Planck-Institut für physiologische und klinische Forschung, W.G. Kerckhoff-Institut, Abteilung Molekulare Zellbiologie, Parkstrasse 1, 61231 Bad Nauheim, Germany.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 5/17/93. Accepted 9/23/93.

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				A. TUFRO, V. F. NORWOOD, R. M. CAREY, and R. A. GOMEZ Vascular Endothelial Growth Factor Induces Nephrogenesis and Vasculogenesis J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2125 - 2134. [Abstract] [Full Text]

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				W. G. Mayhan VEGF increases permeability of the blood-brain barrier via a nitric oxide synthase/cGMP-dependent pathway Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1148 - C1153. [Abstract] [Full Text] [PDF]

				A. Hartmann, M. Kunz, S. Kostlin, R. Gillitzer, A. Toksoy, E.-B. Brocker, and C. E. Klein Hypoxia-induced Up-Regulation of Angiogenin in Human Malignant Melanoma Cancer Res., April 1, 1999; 59(7): 1578 - 1583. [Abstract] [Full Text] [PDF]

				C. J. Corral, A. Siddiqui, L. Wu, C. L. Farrell, D. Lyons, and T. A. Mustoe Vascular Endothelial Growth Factor Is More Important Than Basic Fibroblastic Growth Factor During Ischemic Wound Healing Arch Surg, February 1, 1999; 134(2): 200 - 205. [Abstract] [Full Text] [PDF]

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				W. G. Roberts, J. Delaat, M. Nagane, S. Huang, W. K. Cavenee, and G. E. Palade Host Microvasculature Influence on Tumor Vascular Morphology and Endothelial Gene Expression Am. J. Pathol., October 1, 1998; 153(4): 1239 - 1248. [Abstract] [Full Text] [PDF]

				B. Witzenbichler, T. Asahara, T. Murohara, M. Silver, I. Spyridopoulos, M. Magner, N. Principe, M. Kearney, J.-S. Hu, and J. M. Isner Vascular Endothelial Growth Factor-C (VEGF-C/VEGF-2) Promotes Angiogenesis in the Setting of Tissue Ischemia Am. J. Pathol., August 1, 1998; 153(2): 381 - 394. [Abstract] [Full Text] [PDF]

				B. Witzenbichler, P. C. Maisonpierre, P. Jones, G. D. Yancopoulos, and J. M. Isner Chemotactic Properties of Angiopoietin-1 and -2, Ligands for the Endothelial-specific Receptor Tyrosine Kinase Tie2 J. Biol. Chem., July 17, 1998; 273(29): 18514 - 18521. [Abstract] [Full Text] [PDF]

				F. Xu and J. W. Severinghaus Rat brain VEGF expression in alveolar hypoxia: possible role in high-altitude cerebral edema J Appl Physiol, July 1, 1998; 85(1): 53 - 57. [Abstract] [Full Text] [PDF]

				L. L. Couper, S. R. Bryant, J. Eldrup-Jorgensen, C. E. Bredenberg, and V. Lindner Vascular Endothelial Growth Factor Increases the Mitogenic Response to Fibroblast Growth Factor-2 in Vascular Smooth Muscle Cells In Vivo via Expression of fms-Like Tyrosine Kinase-1 Circ. Res., December 19, 1997; 81(6): 932 - 939. [Abstract] [Full Text]

				S. Soker, S. Gollamudi-Payne, H. Fidder, H. Charmahelli, and M. Klagsbrun Inhibition of Vascular Endothelial Growth Factor (VEGF)-induced Endothelial Cell Proliferation by a Peptide Corresponding to the Exon 7-Encoded Domain of VEGF165 J. Biol. Chem., December 12, 1997; 272(50): 31582 - 31588. [Abstract] [Full Text] [PDF]

				L. E. Benjamin and E. Keshet Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors: Induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal PNAS, August 5, 1997; 94(16): 8761 - 8766. [Abstract] [Full Text] [PDF]

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				C. Patterson, Y. Wu, M.-E. Lee, J. D. DeVault, M. S. Runge, and E. Haber Nuclear Protein Interactions with the Human KDR/flk-1 Promoter in Vivo. REGULATION OF Sp1 BINDING IS ASSOCIATED WITH CELL TYPE-SPECIFIC EXPRESSION J. Biol. Chem., March 28, 1997; 272(13): 8410 - 8416. [Abstract] [Full Text] [PDF]

				V. Lindner and M. A. Reidy Expression of VEGF Receptors in Arteries After Endothelial Injury and Lack of Increased Endothelial Regrowth in Response to VEGF Arterioscler. Thromb. Vasc. Biol., November 1, 1996; 16(11): 1399 - 1405. [Abstract] [Full Text]

				Z. Kovacs, K. Ikezaki, K. Samoto, T. Inamura, M. Fukui, T. Kawamata, and S. P. Finklestein VEGF and flt: Expression Time Kinetics in Rat Brain Infarct Stroke, October 1, 1996; 27(10): 1865 - 1873. [Abstract] [Full Text]

				S. Soker, H. Fidder, G. Neufeld, and M. Klagsbrun Characterization of Novel Vascular Endothelial Growth Factor (VEGF) Receptors on Tumor Cells That Bind VEGF[IMAGE] via Its Exon 7-encoded Domain J. Biol. Chem., March 8, 1996; 271(10): 5761 - 5767. [Abstract] [Full Text] [PDF]

				Y. J. Lee, S. S. Galoforo, C. M. Berns, G. Erdos, A. K. Gupta, D. K. Ways, and P. M. Corry Effect of Ionizing Radiation on AP-1 Binding Activity and Basic Fibroblast Growth Factor Gene Expression in Drug-sensitive Human Breast Carcinoma MCF-7 and Multidrug-resistant MCF-7/ADR Cells J. Biol. Chem., December 1, 1995; 270(48): 28790 - 28796. [Abstract] [Full Text] [PDF]

				J. Muhlhauser, M. J. Merrill, R. Pili, H. Maeda, M. Bacic, B. Bewig, A. Passaniti, N. A. Edwards, R. G. Crystal, and M. C. Capogrossi VEGF165 Expressed by a Replication-Deficient Recombinant Adenovirus Vector Induces Angiogenesis In Vivo Circ. Res., December 1, 1995; 77(6): 1077 - 1086. [Abstract] [Full Text]

				E. Ikeda, M. G. Achen, G. Breier, and W. Risau Hypoxia-induced Transcriptional Activation and Increased mRNA Stability of Vascular Endothelial Growth Factor in C6 Glioma Cells J. Biol. Chem., August 25, 1995; 270(34): 19761 - 19766. [Abstract] [Full Text] [PDF]

				W. Roberts and G. Palade Increased microvascular permeability and endothelial fenestration induced by vascular endothelial growth factor J. Cell Sci., January 6, 1995; 108(6): 2369 - 2379. [Abstract] [PDF]

				M. E. Baldwin, B. Catimel, E. C. Nice, S. Roufail, N. E. Hall, K. L. Stenvers, M. J. Karkkainen, K. Alitalo, S. A. Stacker, and M. G. Achen The Specificity of Receptor Binding by Vascular Endothelial Growth Factor-D Is Different in Mouse and Man J. Biol. Chem., May 25, 2001; 276(22): 19166 - 19171. [Abstract] [Full Text] [PDF]

				H. Su, R. Lu, and Y. W. Kan Adeno-associated viral vector-mediated vascular endothelial growth factor gene transfer induces neovascular formation in ischemic heart PNAS, December 5, 2000; 97(25): 13801 - 13806. [Abstract] [Full Text] [PDF]