This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Costa, M. Articles by Toniolo, P. Search for Related Content PubMed PubMed Citation Articles by Costa, M. Articles by Toniolo, P.

DNA-Protein Cross-Links in Welders: Molecular Implications¹

Nelson Institute of Environmental Medicine, New York University Medical Center, New York, New York 10016

A new method for detecting DNA-protein cross-links involving selective precipitation of DNA containing cross-linked proteins by K⁺-sodium dodecyl sulfate was utilized in the peripheral WBC of 21 male metal are welders and in 26 male controls of similar age and racial characteristics who were not exposed to welding fumes. DNA was quantitated by Hoechst fluorescence. Although the concentration of nickel and chromium in the peripheral blood did not differ between subjects in the two groups, one-fourth of the welders had levels of DNA-protein cross-links that were above the upper limit of the controls. Mean cross-link values were 1.85 ± 1.14% (SD) among the welders and 1.17 ± 0.46% among the controls, a 58% statistically significant difference (P = 0.01). Thus, many welders appeared to be burdened with an excess of DNA-protein cross-links, suggesting exposure to cross-linking agents and, possibly, a detectable biological effect of potential genotoxic consequences.

¹ This work was supported by Grant ES 04895, ES 04715, ES 05512 and ES 00260 from the National Institute of Environmental Health Sciences and by Grant R 814702 from the United States Environmental Protection Agency.

² To whom requests for reprints should be addressed.

Received 10/12/92. Accepted 12/ 4/92.

This article has been cited by other articles:

				Z. Kirpnick-Sobol, R. Reliene, and R. H. Schiestl Carcinogenic Cr(VI) and the Nutritional Supplement Cr(III) Induce DNA Deletions in Yeast and Mice. Cancer Res., April 1, 2006; 66(7): 3480 - 3484. [Abstract] [Full Text] [PDF]

				S. G. Han, Y. Kim, M. L. Kashon, D. L. Pack, V. Castranova, and V. Vallyathan Correlates of Oxidative Stress and Free-Radical Activity in Serum from Asymptomatic Shipyard Welders Am. J. Respir. Crit. Care Med., December 15, 2005; 172(12): 1541 - 1548. [Abstract] [Full Text] [PDF]

				M. Goulart, M.C. Batoreu, A.S. Rodrigues, A. Laires, and J. Rueff Lipoperoxidation products and thiol antioxidants in chromium exposed workers Mutagenesis, September 1, 2005; 20(5): 311 - 315. [Abstract] [Full Text] [PDF]

				M.G. Medeiros, A.S. Rodrigues, M.C. Batoreu, A. Laires, J. Rueff, and A. Zhitkovich Elevated levels of DNA-protein crosslinks and micronuclei in peripheral lymphocytes of tannery workers exposed to trivalent chromium Mutagenesis, January 1, 2003; 18(1): 19 - 24. [Abstract] [Full Text] [PDF]

				F.-Y. Wu, P.-W. Chang, C.-C. Wu, and H.-W. Kuo Correlations of Blood Lead with DNA-Protein Cross-Links and Sister Chromatid Exchanges in Lead Workers Cancer Epidemiol. Biomarkers Prev., March 1, 2002; 11(3): 287 - 290. [Abstract] [Full Text] [PDF]

				N.J. Hodges, B. Adam, A.J. Lee, H.J. Cross, and J.K. Chipman Induction of DNA-strand breaks in human peripheral blood lymphocytes and A549 lung cells by sodium dichromate: association with 8-oxo-2-deoxyguanosine formation and inter-individual variability Mutagenesis, November 1, 2001; 16(6): 467 - 474. [Abstract] [Full Text] [PDF]

				A. Matsumoto and P. C. Hanawalt Histone H3 and Heat Shock Protein GRP78 Are Selectively Cross-Linked to DNA by Photoactivated Gilvocarcin V in Human Fibroblasts Cancer Res., July 1, 2000; 60(14): 3921 - 3926. [Abstract] [Full Text]