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Heterogeneity of Mucin Gene Expression in Normal and Neoplastic Tissues¹

Departments of Medicine and Pathology, University of Minnesota and Veterans Affairs Medical Center, Minneapolis, Minnesota 55417 [S. B. H., G. A. N., C. L., D. L. C.], and Departments of Medicine and Pathology, University of California and Veterans Affairs Medical Center, San Francisco, California 94121 [P. S. Y., E. T. G., R. D., Y. S. K.]

To determine the relative expression of distinct mucin genes in normal and neoplastic tissue, antibodies and cDNA probes that recognize the core tandem repeat sequences of membrane-bound (MUC1) and secreted (MUC2 and MUC3) mucins were used for immunohistochemical and RNA Northern and slot-blot analysis. MUC1 mRNA was detected in all epithelial tissues tested. MUC1 core peptide, recognized by monoclonal antibodies 139H2 and DF3, was highly expressed on apical membranes of bronchus, breast, salivary gland, pancreas, prostate, and uterus, and was sparsely expressed in gastric surface cells, gallbladder, small intestine, and colonic epithelium. In contrast, MUC2 and MUC3 gene expression was primarily restricted to the intestinal tract. MUC2 mRNA was highly expressed in normal jejunum, ileum, and colon, compared with very low levels in normal bronchus and gallbladder. MUC3 mRNA was highly expressed in normal jejunum, ileum, colon, and gallbladder. Immunohistochemical studies using antibodies against synthetic MUC2 (anti-MRP) and MUC3 (anti-M3P) peptides indicate that MUC2- and MUC3-producing cells in the gastrointestinal tract are distinct. Goblet cells of the small intestine and colon reacted strongly with anti-MRP, whereas M3P reactivity was restricted to columnar cells of small intestinal villi, surface colonic epithelium, and gallbladder. Mucin protein epitopes and mRNA levels were frequently altered in adenocarcinomas compared to corresponding normal tissues. Alterations included increased expression, aberrant expression, and, less frequently, loss of expression. Increased MUC1 immunoreactivity was observed in most adenocarcinomas of the breast, lung, stomach, pancreas, prostrate, and ovary. In addition, with the exception of prostrate cancer, focal aberrant expression of MUC2 and MUC3 epitopes was frequently observed. Increased MUC1, MUC2, and MUC3 epitopes were present in colon adenocarcinomas of all histological subtypes, with the greatest increase of MUC2 epitopes observed in colloid (mucinous) colon cancers. MUC2 or MUC3 mRNA levels were increased in colloid colon cancer compared with normal colon, however in well- and moderately well-differentiated colon cancers MUC1, 2 and 3 mRNA levels were decreased. Compared with corresponding normal tissue, MUC1 mRNA levels were increased in breast cancer and well-differentiated lung cancers, and MUC3 mRNA was increased in gastric adenocarcinomas. Normal stomach lacked both MUC2 and MUC3 immunoreactivity and mRNA, however, MUC2 and MUC3 proteins and mRNA were highly expressed in gastric intestinal metaplasia. In conclusion, mucin genes are independently regulated and their expression is organ- and cell type-specific. Furthermore, neoplastic transformation is associated with dysregulated expression of both membrane-bound and secreted mucin core protein epitopes and may be due to altered mucin mRNA levels and/or altered mucin glycosylation.

¹ This work was supported by a Veterans Administration Associate Investigator Award and Research Advisory Group Grant (S. B. H.), a Veterans Administration Medical Investigator Award (Y. S. K.), and the Research Service of the Veterans Administration.

² To whom requests for reprints should be addressed, at Department of Gastroenterology (111-D), Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417.

Received 7/ 3/92. Accepted 11/13/92.

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				P. de Cremoux, J. M. Extra, M. G. Denis, J. Y. Pierga, E. Bourstyn, C. Nos, K. B. Clough, E. Boudou, E. C. Martin, A. Müller, et al. Detection of MUC1-expressing Mammary Carcinoma Cells in the Peripheral Blood of Breast Cancer Patients by Real-Time Polymerase Chain Reaction Clin. Cancer Res., August 1, 2000; 6(8): 3117 - 3122. [Abstract] [Full Text]

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				G R Van den Brink, K M A J Tytgat, R W M Van der Hulst, C M Van der Loos, A W C Einerhand, H A Buller, and J Dekker H pylori colocalises with MUC5AC in the human stomach Gut, May 1, 2000; 46(5): 601 - 607. [Abstract] [Full Text] [PDF]

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				M P Buisine, L Devisme, T C Savidge, C Gespach, B Gosselin, N Porchet, and J P Aubert Mucin gene expression in human embryonic and fetal intestine Gut, October 1, 1998; 43(4): 519 - 524. [Abstract] [Full Text] [PDF]

				P. Sharma, L. Dudus, P. A. Nielsen, H. Clausen, J. R. Yankaskas, M. A. Hollingsworth, and J. F. Engelhardt MUC5B and MUC7 Are Differentially Expressed in Mucous and Serous Cells of Submucosal Glands in Human Bronchial Airways Am. J. Respir. Cell Mol. Biol., July 1, 1998; 19(1): 30 - 37. [Abstract] [Full Text]

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				M P Buisine, J F Colombel, M Lecomte-Houcke, P Gower, J P Aubert, N Porchet, and A Janin Abnormal mucus in cap polyposis Gut, January 1, 1998; 42(1): 135 - 138. [Abstract] [Full Text] [PDF]

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				J. R. Gum Jr., J. J. L. Ho, W. S. Pratt, J. W. Hicks, A. S. Hill, L. E. Vinall, A. M. Roberton, D. M. Swallow, and Y. S. Kim MUC3 Human Intestinal Mucin. ANALYSIS OF GENE STRUCTURE, THE CARBOXYL TERMINUS, AND A NOVEL UPSTREAM REPETITIVE REGION J. Biol. Chem., October 17, 1997; 272(42): 26678 - 26686. [Abstract] [Full Text] [PDF]

				M. D. Burdick, A. Harris, C. J. Reid, T. Iwamura, and M. A. Hollingsworth Oligosaccharides Expressed on MUC1 Produced by Pancreatic and Colon Tumor Cell Lines J. Biol. Chem., September 26, 1997; 272(39): 24198 - 24202. [Abstract] [Full Text] [PDF]

				A. Velcich, L. Palumbo, L. Selleri, G. Evans, and L. Augenlicht Organization and Regulatory Aspects of the Human Intestinal Mucin Gene (MUC2) Locus J. Biol. Chem., March 21, 1997; 272(12): 7968 - 7976. [Abstract] [Full Text] [PDF]

				R. A. Pimental, J. Julian, S. J. Gendler, and D. D. Carson Synthesis and Intracellular Trafficking of Muc-1 and Mucins by Polarized Mouse Uterine Epithelial Cells J. Biol. Chem., November 8, 1996; 271(45): 28128 - 28137. [Abstract] [Full Text] [PDF]

				A. Bergeron, S. Champetier, Hélèn. LaRue, and Y. Fradet MAUB Is a New Mucin Antigen Associated with Bladder Cancer J. Biol. Chem., March 22, 1996; 271(12): 6933 - 6940. [Abstract] [Full Text] [PDF]

				T.éc. Lesuffleur, F. Roche, A. S. Hill, M. Lacasa, M. Fox, D. M. Swallow, A. Zweibaum, and F. X. Real Characterization of a Mucin cDNA Clone Isolated from HT-29 Mucus-secreting Cells J. Biol. Chem., June 9, 1995; 270(23): 13665 - 13673. [Abstract] [Full Text] [PDF]

				J. Chambers, M. Hollingsworth, A. Trezise, and A Harris Developmental expression of mucin genes MUC1 and MUC2 J. Cell Sci., January 2, 1994; 107(2): 413 - 424. [Abstract] [PDF]

				D. L. Witsell, M. C. Weissler, E. Livanos, C. Bova-Hill, and T. M. Gilmer Unique Chromosomal Rearrangement and Mucin Production in a Novel Salivary Myoepithelial Cell Strain Arch Otolaryngol Head Neck Surg, October 1, 1993; 119(10): 1151 - 1157. [Abstract] [PDF]

				T Lesuffleur, N Porchet, J. Aubert, D Swallow, J. Gum, Y. Kim, F. Real, and A Zweibaum Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT-29 cell subpopulations J. Cell Sci., January 11, 1993; 106(3): 771 - 783. [Abstract] [PDF]

				A. Choudhury, R. K. Singh, N. Moniaux, T. H. El-Metwally, J.-P. Aubert, and S. K. Batra Retinoic Acid-dependent Transforming Growth Factor-beta 2-mediated Induction of MUC4 Mucin Expression in Human Pancreatic Tumor Cells Follows Retinoic Acid Receptor-alpha Signaling Pathway J. Biol. Chem., October 20, 2000; 275(43): 33929 - 33936. [Abstract] [Full Text] [PDF]

				M. Perrais, P. Pigny, M.-P. Buisine, N. Porchet, J.-P. Aubert, and I. Van Seuningen-Lempire Aberrant Expression of Human Mucin Gene MUC5B in Gastric Carcinoma and Cancer Cells. IDENTIFICATION AND REGULATION OF A DISTAL PROMOTER J. Biol. Chem., April 27, 2001; 276(18): 15386 - 15396. [Abstract] [Full Text] [PDF]