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British Columbia Cancer Research Center, Vancouver V5Z IL3 [P. L. O., R. E. D.], and British Columbia Cancer Agency, Vancouver V5Z 4E6 [J. L., I. A. O., S. M. J.], British Columbia, Canada
A new gel electrophoresis method has been used to quantify hypoxic fraction in human tumors. Radiation-induced DNA damage was measured in individual tumor cells, where the radiobiologically hypoxic cells were observed as a subpopulation showing a 3-fold reduction in DNA strand breaks. Patients receiving palliative radiotherapy for breast cancers were given a single dose of 5–10 Gy, and a fine needle aspiration biopsy was taken immediately after irradiation. Hypoxic cells were detected in seven of eight tumors. In four tumors, bivariate analyses of DNA content versus DNA damage to individual cells allowed distinction between the response of diploid normal cells and aneuploid tumor cells. These early results indicate that "comet assay" shows considerable promise for resolving the extent and significance of hypoxia in human tumors.
1 This work was supported by Grant CA-37879 awarded by the USPHS, Department of Health and Human Services.
2 To whom requests for reprints should be addressed, at the Medical Biophysics Department, British Columbia Cancer Research Centre, 601 W. 10th Ave., Vancouver, British Columbia, Canada V5Z IL3.
Received 12/ 2/92. Accepted 12/30/92.
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