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The Two Major CD44 Proteins Expressed on a Metastatic Rat Tumor Cell Line Are Derived from Different Splice Variants: Each One Individually Suffices to Confer Metastatic Behavior¹

Kernforschungszentrum Karlsruhe, Institut für Genetik, D-7500 Karlsruhe 1 [W. R., M. H., K-H. H., H. P., P. H.], and Deutsches Krebsforschungszentrum Heidelberg, Institut für Immunologie [R. S-A.] und für Radiologie und Pathophysiologie [M. Z.], Im Neuenheimer Feld 280, D-6900 Heidelberg, 1, Germany

The metastatic pancreas carcinoma cell line BSp73ASML produces a variety of different splice variants of the transmembrane glycoprotein CD44. The NH₂-terminal portions are identical and heavily glycosylated. The variant sequences are inserted just outside the transmembrane region of the molecules. The two most abundant variants have 162 and 85 extra amino acids, respectively. When individually expressed, these suffice to establish metastatic properties in the nonmetastatic tumor cell line BSp73AS, as assayed by the spontaneous metastasis protocol.

¹ Supported by Deutsche Forschungsgemeinschaft Grant He551/7-1. W. R. has been a recipient of a stipend of the Graduiertenförderung of Baden-Württemberg. K. H. H. receives a fellowship from the Deutsche Forschungsgemeinschaft.

² Present address: Forschungsinstitut für Molekulare Pathologie, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.

³ To whom requests for reprints should be addressed, at Kernforschungszentrum, Karlsruhe, Institut für Genetik, 7514 Eggenstein-Leopoldshafen, Germany.

Received 6/12/92. Accepted 1/11/93.

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