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Sequence Specificity in the Interaction of the Four Stereoisomeric Benzo[c]phenanthrene Dihydrodiol Epoxides with the supF Gene¹

Chemistry of Carcinogenesis Laboratory [H. R., C. A. H. B., A. D.], ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, and Laboratory of Bioorganic Chemistry [H. Y., D. M. J.], National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892

The shuttle vector pS189 was treated with each of the four configurational isomers of benzo[c]phenanthrene 3,4-dihydrodiol 1,2-epoxide, and the modified DNA was used as a template in a polymerase arrest assay examining the supF gene. Sites at which polymerase (Sequenase, version 2.0) progress along the template was blocked were presumed to be at or near sites of adduct formation. The polymerase arrest sites were compared with recently reported mutation hotspots induced by these agents in this gene (Bigger et al., Proc. Natl. Acad. Sci. USA, 89: 368–372, 1992). For 31 of 32 mutation hotspots, a polymerase arrest band was present at or 1 or 2 nucleotides 3'- to that site, indicating that adduct formation tended to be associated with mutation hotspots. However, the arrest bands near mutation hotspots were not particularly prominent in all cases, and there were many sites of substantial polymerase arrest that were not in the vicinity of mutation hotspots. Thus, factors in addition to chemical selectivity must play key roles in determining sites of mutation.

¹ Research sponsored in part by the National Cancer Institute, DHHS, under contract NO1-CO-74101 with ABL.

² Present address: Microbiological Associates, 9900 Blackwell Road, Rockville, MD 20850.

³ To whom requests for reprints should be addressed.

Received 10/ 5/92. Accepted 1/11/93.

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