| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Jefferson Cancer Institute, Thomas Jefferson Medical College, Philadelphia, Pennsylvania 19107
Protein tyrosine phosphatases, on purely theoretical grounds, were suggested as possible tumor suppressor genes, and receptor protein tyrosine phosphatase 
(PTPRG) has been proposed, on the basis of its location at human chromosome region 3p14.2, specifically as a tumor suppressor gene for renal cell carcinoma.
We have isolated murine genomic and complementary DNA clones for analysis and mapping of the murine Ptprg locus; interspecific backcross analysis showed that the Ptprg locus maps to the centromeric region of mouse chromosome 14. We also observed a homozygous, intragenic deletion in the Ptprg gene in all clonal derivatives of the original L-cell strain, a methylcholanthrene-treated mouse connective tissue cell line which produces sarcomas in syngeneic mice. The deletion begins in the second intron of the carbonic anhydrase-like domain of the Ptprg gene and ends in the fourth intron of the carbonic anhydrase-like domain. At the genomic level, perhaps several hundred kilobases of DNA are deleted; at the complementary DNA level the 400 base pairs comprising exons 2, 3, and 4 of the carbonic anhydrase-like domain are deleted. By reverse transcription polymerase chain reaction, an amplified fragment is produced from L-cell mRNA which is 400 base pairs shorter than the wild type gene product, suggesting that the deleted gene is transcribed and may produce a protein product.
Thus, mouse L-cells have lost one Ptprg allele and sustained an intragenic deletion in the other; such allele loss and mutation frequently occur at tumor suppressor gene loci.
1 This work was supported by USPHS Grant CA 21124 and by an American Cancer Society Junior Faculty Research Award (L. D. S.).
2 To whom requests for reprints should be addressed, at Jefferson Cancer Institute, Thomas Jefferson Medical College, BLSB, Rm. 1008A, 233 S. 10th St., Philadelphia, PA 19107.
Received 2/ 8/93. Accepted 2/26/93.
This article has been cited by other articles:
|
|
 |
|
  O. A. Hampton, P. Den Hollander, C. A. Miller, D. A. Delgado, J. Li, C. Coarfa, R. A. Harris, S. Richards, S. E. Scherer, D. M. Muzny, et al. A sequence-level map of chromosomal breakpoints in the MCF-7 breast cancer cell line yields insights into the evolution of a cancer genome Genome Res., February 1, 2009; 19(2): 167 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Adhami, N. Ahmad, and H. Mukhtar Molecular Targets for Green Tea in Prostate Cancer Prevention J. Nutr., July 1, 2003; 133(7): 2417S - 2424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ulmasov, A. Waheed, G. N. Shah, J. H. Grubb, W. S. Sly, C. Tu, and D. N. Silverman Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers PNAS, December 19, 2000; 97(26): 14212 - 14217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Tureci, U. Sahin, E. Vollmar, S. Siemer, E. Gottert, G. Seitz, A.-K. Parkkila, G. N. Shah, J. H. Grubb, M. Pfreundschuh, et al. Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers PNAS, June 23, 1998; 95(13): 7608 - 7613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Barford, A. Flint, and N. Tonks Crystal structure of human protein tyrosine phosphatase 1B Science, March 11, 1994; 263(5152): 1397 - 1404. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
