This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bidwell, J. P. Articles by Stein, G. S. Search for Related Content PubMed PubMed Citation Articles by Bidwell, J. P. Articles by Stein, G. S.

Nuclear Matrix Proteins Distinguish Normal Diploid Osteoblasts from Osteosarcoma Cells¹

Department of Cell Biology, University of Massachusetts, Worcester, Massachusetts 01655 [E. G. F., A. van W., J. L. S., J. B. L., G. S. S.]; Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 [S. P]; and Departments of Anatomy and Periodontics, Indiana University Schools of Medicine and Dentistry, Indianapolis, Indiana 46202 [J. P. B.]

Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with those in normal diploid osteoblasts. The tumor-derived cells coexpress genes which are expressed in a sequential and mutually exclusive manner during the progressive stages of osteoblast differentiation. In osteosarcoma cells two-dimensional electrophoretic analysis indicates a composite representation of nuclear matrix proteins characteristic of both the proliferative and postproliferative periods of osteoblast phenotype development. In addition, nuclear matrix proteins unique to the tumor cells and the absence of nuclear matrix proteins found only in normal diploid osteoblasts are observed. Tumor-specific nuclear matrix proteins include those expressed in a proliferation-dependent and independent manner. There is a parallel relationship between nuclear matrix proteins and the expression of cell growth and tissue-specific genes during osteoblast differentiation and in osteosarcoma cells where the developmental sequence of gene expression has been abrogated. Nuclear matrix proteins therefore provide markers reflecting defined periods of bone cell differentiation and phenotypic characteristics of an osteosarcoma.

¹ Supported by grants AR33920, AR35166, AR39588, and GM32010 from the NIH.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 10/ 5/93. Accepted 11/16/93.

This article has been cited by other articles:

				J. Sagara, T. Higuchi, Y. Hattori, M. Moriya, H. Sarvotham, H. Shima, H. Shirato, K. Kikuchi, and S. Taniguchi Scapinin, a Putative Protein Phosphatase-1 Regulatory Subunit Associated with the Nuclear Nonchromatin Structure J. Biol. Chem., November 14, 2003; 278(46): 45611 - 45619. [Abstract] [Full Text] [PDF]

				J. Pratap, M. Galindo, S. K. Zaidi, D. Vradii, B. M. Bhat, J. A. Robinson, J.-Y. Choi, T. Komori, J. L. Stein, J. B. Lian, et al. Cell Growth Regulatory Role of Runx2 during Proliferative Expansion of Preosteoblasts Cancer Res., September 1, 2003; 63(17): 5357 - 5362. [Abstract] [Full Text] [PDF]

				K. S. Harrington, A. Javed, H. Drissi, S. McNeil, J. B. Lian, J. L. Stein, A. J. van Wijnen, Y.-L. Wang, and G. S. Stein Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains J. Cell Sci., November 1, 2002; 115(21): 4167 - 4176. [Abstract] [Full Text] [PDF]

				G. S. Stein, M. Montecino, A. J. van Wijnen, J. L. Stein, and J. B. Lian Nuclear Structure-Gene Expression Interrelationships: Implications for Aberrant Gene Expression in Cancer Cancer Res., April 1, 2000; 60(8): 2067 - 2076. [Abstract] [Full Text]

				S. McNeil, C. Zeng, K. S. Harrington, S. Hiebert, J. B. Lian, J. L. Stein, A. J. van Wijnen, and G. S. Stein The t(8;21) chromosomal translocation in acute myelogenous leukemia modifies intranuclear targeting of the AML1/CBFalpha 2 transcription factor PNAS, December 21, 1999; 96(26): 14882 - 14887. [Abstract] [Full Text] [PDF]

				G. S. STEIN, A. J. VAN WIJNEN, J. L. STEIN, J. B. LIAN, S. H. POCKWINSE, and S. MCNEIL Implications for interrelationships between nuclear architecture and control of gene expression under microgravity conditions FASEB J, May 1, 1999; 13(9001): 157 - 166. [Abstract] [Full Text] [PDF]

				J. Choi, A. van Wijnen, F Aslam, J. Leszyk, J. Stein, G. Stein, J. Lian, and S Penman Developmental association of the beta-galactoside-binding protein galectin-1 with the nuclear matrix of rat calvarial osteoblasts J. Cell Sci., January 10, 1998; 111(20): 3035 - 3043. [Abstract] [PDF]

				J. D. McCaffery, M. Gapany, R. A. Faust, A. T. Davis, G. L. Adams, and K. Ahmed Nuclear Matrix Proteins as Malignant Markers in Squamous Cell Carcinoma of the Head and Neck Arch Otolaryngol Head Neck Surg, March 1, 1997; 123(3): 283 - 288. [Abstract] [PDF]

				M. Alvarez, H. Long, J. Onyia, J. Hock, W. Xu, and J. Bidwell Rat Osteoblast and Osteosarcoma Nuclear Matrix Proteins Bind with Sequence Specificity to the Rat Type I Collagen Promoter Endocrinology, January 1, 1997; 138(1): 482 - 489. [Abstract] [Full Text] [PDF]

				S. M. Hawkins, T. Kohwi-Shigematsu, and D. G. Skalnik The Matrix Attachment Region-binding Protein SATB1 Interacts with Multiple Elements within the gp91phox Promoter and Is Down-regulated during Myeloid Differentiation J. Biol. Chem., November 21, 2001; 276(48): 44472 - 44480. [Abstract] [Full Text] [PDF]