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Elongation Factor 1 Is Enhanced following Exposure to Ionizing Radiation¹

Department of Radiation Medicine, Georgetown University School of Medicine, Washington, DC 20007

We have used the technique of differential display of mRNA to investigate gene expression patterns in a human squamous carcinoma cell line, SCC-35, following exposure to ionizing radiation. We observed that elongation factor 1 (EF-1) expression increased within 2 h of radiation exposure with maximum expression at 8 h. These data identify EF-1 as a radiation-inducible gene. Cell cycle analysis shows that EF-1 expression enhancement precedes G₂ arrest in these cells, suggesting that EF-1 may participate in the G₂-M cell cycle checkpoint.

¹ This work was supported by NIH Grants CA45408 (A. D.) and CA63023 (M. J.).

² To whom requests for reprints should be addressed, at Georgetown University Medical Center, V. T. Lombardi Cancer Research Center, Department of Radiation Medicine, 3800 Reservoir Road, N. W., Washington, DC 20007-2197.

Received 2/ 1/94. Accepted 4/ 6/94.

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