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Up-Regulation of a Mutant Form of p53 by Doxorubicin in Human Squamous Carcinoma Cells

Joint Center for Radiation Therapy [T. T. K., L. M-B.] and Laboratory of Gynecologic Oncology, Department of Obstetrics, Gynecology, and Reproductive Biology [C. H. M.], Harvard Medical School, Boston, Massachusetts 02115

Human squamous carcinoma A431 cells express a high level of epidermal growth factor (EGF) receptor. The cells carry only a mutated form of the p53 gene, the GA mutation at codon 273 which results in an arginine to histidine substitution (mp53). The temporal changes of EGF receptor, c-Raf-1, mp53, and cell cycle distribution in A431 cells after 1-h exposure to doxorubicin (DOX) are examined. EGF receptor in A431 cells is inactivated at 5 min; subsequently, the receptor level increases and reaches its maximum 4–8 h after DOX treatment. Dephosphorylation of c-Raf-1 is detected at 30 min and the decay of the protein is demonstrated at 8 h in cells after exposure to DOX. The level of mp53 in A431 cells remains unchanged for 8 h after DOX treatment but increases by about 20-fold at 24 h. There is no significant change in cell cycle distribution in A431 cells for up to 8 h after DOX exposure, whereas cells are accumulated in S and G₂-M phases by 24 h. It is postulated that DOX inactivates EGF signal transduction and induces mp53. The increase in mp53 is coincident with DOX-induced G₂-M block in cells.

¹ To whom requests for reprints should be addressed, at Joint Center for Radiation Therapy, Harvard Medical School, 50 Binney St., Boston, MA 02115.

Received 2/18/94. Accepted 4/21/94.

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