This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bérubé, N. G. Articles by Chevrette, M. Search for Related Content PubMed PubMed Citation Articles by Bérubé, N. G. Articles by Chevrette, M.

Suppression of Tumorigenicity of Human Prostate Cancer Cells by Introduction of Human Chromosome del(12)(q13)¹

Department of Biochemistry, Faculty of Medicine, University of Ottawa, Ontario K1H 8M5, Canada

The introduction of normal chromosomes into tumor cells by microcell fusion-mediated transfer is a powerful technique to identify putative tumor suppressor genes. We have used this approach to independently transfer human chromosomes 3 and 12 into a human prostate cancer cell line, DU 145. We showed that while the extra copy of chromosome 3 had no effect on the in vivo tumorigenicity of these cells, microcell hybrids containing an introduced portion of chromosome 12 (12pter-12q13) exhibited complete suppression of tumorigenicity in athymic nude mice. The presence of a dual selectable marker facilitated the selection for cells having segregated del(12)(q13). Loss of this fragment in three different clones led to reexpression of the malignant phenotype. These results demonstrate that one or more genes on human chromosome 12 function as tumor suppressors of prostate carcinogenesis.

¹ This work was supported by a grant from the Cancer Research Society, Montréal, Québec, Canada. N. G. B. and M. D. S. are recipients of Ontario Graduate Scholarships.

² To whom requests for reprints should be addressed, at Department of Biochemistry, University of Ottawa, 451 Smyth Road, Ottawa, Ontario K1H 8M5, Canada.

Received 2/ 4/94. Accepted 5/ 3/94.

This article has been cited by other articles:

				J.-C. Wang, L. R. Begin, N. G. Berube, S. Chevalier, A. G. Aprikian, H. Gourdeau, and M. Chevrette Down-Regulation of CD9 Expression during Prostate Carcinoma Progression Is Associated with CD9 mRNA Modifications Clin. Cancer Res., April 15, 2007; 13(8): 2354 - 2361. [Abstract] [Full Text] [PDF]

				B. A. Yoshida, M. M. Sokoloff, D. R. Welch, and C. W. Rinker-Schaeffer Metastasis-Suppressor Genes: a Review and Perspective on an Emerging Field J Natl Cancer Inst, November 1, 2000; 92(21): 1717 - 1730. [Abstract] [Full Text] [PDF]

				N. Saadane, L. Alpert, and L. E. Chalifour Altered molecular response to adrenoreceptor-induced cardiac hypertrophy in Egr-1-deficient mice Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H796 - H805. [Abstract] [Full Text] [PDF]

				G. P. Jarvik, J. L. Stanford, E. L. Goode, R. McIndoe, S. Kolb, M. Gibbs, L. Hood, and E. A. Ostrander Confirmation of Prostate Cancer Susceptibility Genes Using High-Risk Families J Natl Cancer Inst Monographs, December 1, 1999; 1999(26): 81 - 87. [Abstract] [Full Text]

				M. I. Gallego and P. A. Lazo Deletion in Human Chromosome Region 12q13-15 by Integration of Human Papillomavirus DNA in a Cervical Carcinoma Cell Line J. Biol. Chem., October 13, 1995; 270(41): 24321 - 24326. [Abstract] [Full Text] [PDF]