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A Negative Regulatory Factor Is Missing in a Human Metastatic Breast Cancer Cell Line¹

Department of Biochemistry and Molecular Biophysics, and Massey Cancer Center, Medical College of Virginia/Virginia Commonwealth University, Richmond, Virginia 23298

The intermediate filament protein, vimentin, is differentially expressed in various tissues and stages of development and in metastatic versus nonmetastatic breast cancer cell lines. Previously, we have shown vimentin expression to be regulated at least in part by a silencer element which binds a M_r 95,000 protein and an overriding, antisilencer element which binds a M_r 140,000 protein. Southwestern blot (DNA-protein) analyses indicate that silencer protein binding activity is missing in the metastatic breast cancer cell line (MDA-MB-231), where vimentin is highly expressed, but is present in the nonmetastatic breast cancer cell line, MCF-7, where vimentin is not expressed. This suggests that the absence of a functional silencer protein may lead to expression of vimentin as well as other genes which contribute to the metastatic state.

¹ This work was supported in part by USPHS Grant HL45422 to Z. E. Z.

² To whom requests for reprints should be addressed, at Department of Biochemistry and Molecular Biophysics, Box 980614 MCV Station, Virginia Commonwealth University, Richmond, VA 23298.

Received 2/ 4/94. Accepted 5/ 3/94.

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