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Determination of Local Oxygen Consumption Rates in Tumors¹

Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710 [M. W. D., E. T. O.], and Department of Physiology, University of Arizona, Tucson, Arizona 85724 [T. W. S., R. H., J. F. G.]

At any location in a respiring tissue, partial pressure of oxygen (PO₂) is influenced by the local oxygen consumption rate. Consumption rates in vascular tumor tissues have previously been estimated for macroscopic regions. Using oxygen electrodes, we measured PO₂ profiles across microregions (87 µm to 286 µm wide) of tumors (R3230AC mammary adenocarcinoma) in a rat dorsal skin flap preparation and mapped adjacent microvessels. By comparing measured PO₂ values with theoretical simulations, we deduced local consumption rates. Results for six profiles ranged from 0.83 to 2.22 cm³ O₂/100 g/min. The mean (± SD) was 1.52 ± 0.51 cm³ O₂/100 g/min. This technique permits investigation of variations in consumption at a microregional level.

¹ This work was supported by NIH-NCI Grant CA40355 and NIH-HLBI Grant HL07249.

² To whom requests for reprints should be addressed, at Department of Radiation Oncology, Tumor Microcirculation Laboratory, Duke University Medical Center, Box 3455, Durham, NC 27710.

Received 12/21/93. Accepted 5/17/94.

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