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Secretion of Bioactive Granulocyte-Macrophage Colony-stimulating Factor by Human Colorectal Carcinoma Cells¹

Department of Cellular Biology, Swiss Institute for Experimental Cancer Research [H. L., J. W., A. Y., N. O.], and Institute of Biochemistry, University of Lausanne [S. H.], Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland; Department of Medical Oncology, Thorax Hospital, Amalienstrasse 5, D-69126 Heidelberg, Germany [J. R. F.]; and Department of Surgery, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland [J-C. G.]

Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.

¹ This work was supported by the Swiss National Foundation (Grant 31-28590-90) and Grant FOR.293 from the Ligue Suisse contre le Cancer to H. L.

² To whom requests for reprints should be addressed.

Received 4/14/94. Accepted 6/ 2/94.

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