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Lack of a Correlation between Radiosensitivity and DNA Double-Strand Break Induction or Rejoining in Six Human Tumor Cell Lines¹

British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada V5Z 1L3

The neutral filter elution method and the neutral comet assay have been used to analyze radiation-induced DNA damage and repair in 6 human tumor cell lines: HT-144 melanoma; DU-145 prostate carcinoma; U-87 glioma; WiDr and HT-29 colon adenocarcinomas; and SiHa cervical carcinoma. In spite of large differences in intrinsic radiosensitivity measured using a clonogenic assay, double-strand break induction, rejoining rate, and amount of residual DNA damage 4 h after irradiation were similar among these cell lines when measured using the neutral comet assay. Differences in initial numbers of double-strand breaks were observed using the neutral filter elution method; however, there was no correlation with radiosensitivity, nor did the rejoining rate or amount of residual DNA damage measured using filter elution correlate with radiation sensitivity. We conclude that neither double-strand break assay is able to reliable rank cells according to clonogenic survival following irradiation.

¹ This work was supported by a grant from the National Cancer Institute of Canada with funds provided by the Canadian Cancer Society.

² To whom requests for reprints should be addressed, at the Medical Biophysics Department, British Columbia Cancer Research Centre, 601 W. 10th Ave., Vancouver, British Columbia, Canada, V5Z 1L3.

Received 3/25/94. Accepted 5/17/94.

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