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Immunohistochemical Staining of Human Spermidine/Spermine N¹-Acetyltransferase Superinduced in Response to Treatment with Antitumor Polyamine Analogues¹

The Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231 [R. A. C., E. W. G.], and The Departments of Cellular and Molecular Physiology and Pharmacology, The Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pennsylvania 17033 [A. E. P.]

A superinduction of the polyamine catabolic enzyme, spermidine/spermine N¹-acetyltransferase (SSAT) accompanies the phenotype-specific cytotoxic response to a class of antitumor polyamine analogues in several important human solid tumor models. A highly specific antiserum against the human SSAT protein has been developed. Using this antiserum we demonstrate that polyamine analogue treatment in vitro or in vivo results in an induction of SSAT protein which is uniformly distributed throughout the cytoplasm of treated tumor cells. This new biochemical tool will be useful in the examination of the association of superinduced SSAT activity and cell type-specific cytotoxicity. Additionally, since clinical trials have begun on one of the SSAT-inducing polyamine analogues, this antiserum may be useful as a diagnostic tool in differentiating responsive and nonresponsive tumor cell populations in treated patients.

¹ This work was supported by NIH Grants CA51085, CA58184, and GM26290.

² To whom requests for reprints should be addressed, at The Oncology Center, The Johns Hopkins University School of Medicine, 424 North Bond Street, Baltimore, MD 21231.

Received 6/ 7/94. Accepted 6/29/94.

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