This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Okada, K. Articles by Shiku, H. Search for Related Content PubMed PubMed Citation Articles by Okada, K. Articles by Shiku, H.

Isolation of Human nm23 Genomes and Analysis of Loss of Heterozygosity in Primary Colorectal Carcinomas Using a Specific Genomic Probe¹

Department of Oncology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852 [K. O., T. U., H. B., K. F., H. S.], and The First Department of Surgery, Fukui Medical School, 23-3 Shimoaizuki, Matsuoka-cho, Yoshida-gun, Fukui 910-11 [T. G., A. Y.], Japan

Genomic clones of nm23-H1 and -H2 were isolated. The nm23-H1 and -H2 genes were located in a tandem array 4 kilobases apart. Each genome contained 5 exons and most of the splicing sites in the exon-intron junctions of two isotypes were essentially identical. A probe derived from intron 4 of nm23-H1 was used to examine the loss of heterozygosity (LOH) in primary colorectal carcinomas. Twenty-nine of 42 samples were informative and LOH was found in 3 of these 29. In all three samples with LOH, cancer tissue expressed lower levels of nm23-H1 mRNA when compared with those without LOH.

¹ This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

² To whom requests for reprints should be addressed.

Received 3/28/94. Accepted 6/14/94.

This article has been cited by other articles:

				S Kapitanovic, T Cacev, M Berkovic, M Popovic-Hadzija, S Radosevic, S Seiwerth, S Spaventi, K Pavelic, and R Spaventi nm23-H1 expression and loss of heterozygosity in colon adenocarcinoma J. Clin. Pathol., December 1, 2004; 57(12): 1312 - 1318. [Abstract] [Full Text] [PDF]

				N. Niitsu, J. Okabe-Kado, M. Nakayama, N. Wakimoto, A. Sakashita, N. Maseki, K. Motoyoshi, M. Umeda, and Y. Honma Plasma levels of the differentiation inhibitory factor nm23-H1 protein and their clinical implications in acute myelogenous leukemia Blood, August 1, 2000; 96(3): 1080 - 1086. [Abstract] [Full Text] [PDF]

				N. Niitsu, J. Okabe-Kado, T. Kasukabe, Y. Yamamoto-Yamaguchi, M. Umeda, and Y. Honma Prognostic Implications of the Differentiation Inhibitory Factor nm23-H1 Protein in the Plasma of Aggressive Non-Hodgkin's Lymphoma Blood, November 15, 1999; 94(10): 3541 - 3550. [Abstract] [Full Text] [PDF]

				A. Yokoyama, J. Okabe-Kado, N. Wakimoto, H. Kobayashi, A. Sakashita, N. Maseki, T. Nakamaki, K.-i. Hino, S. Tomoyasu, N. Tsuruoka, et al. Evaluation by Multivariate Analysis of the Differentiation Inhibitory Factor nm23 as a Prognostic Factor in Acute Myelogenous Leukemia and Application to Other Hematologic Malignancies Blood, March 15, 1998; 91(6): 1845 - 1851. [Abstract] [Full Text] [PDF]