| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Medicine, Gastroenterology Division, University of Maryland School of Medicine and Baltimore Veterans Affairs Hospital, Baltimore, Maryland 21201 [H. S., L. T., J. Y., H-Y. J., J. B., G. M. G., J. M. A., S. J. M.] and Department of Pathology, The Mount Sinai School of Medicine of the City University of New York, New York, New York [M. H., G. M. G., N. H.]
Microsatellites are short nucleotide repeat sequences present throughout the human genome. Alterations of microsatellites, comprising extra or missing copies of these sequences, have been termed microsatellite instability. This abnormality occurs in sporadic and hereditary adenocarcinomas of the proximal colon, as well as in many other tumor types. We determined whether microsatellite instability occurred in ulcerative colitis-associated cancers or precancerous dysplasias. Sixty-three patients were evaluated, consisting of 188 samples of genomic DNA (63 normal controls, 68 cancers, 52 dysplasias, and 5 adjacent tissues) at loci D2S119, D2S123, D2S147, D10S197, and D11S904. Multiplex polymerase chain reaction was performed using one radiolabeled nucleotide, and the products were electrophoresed on denaturing polyacrylamide gels. Seventeen of the 63 patients (27%) possessed lesions showing instability at 1 or more loci. Fourteen of 68 tumor samples (21%) and ten of 52 dysplasias (19%) displayed instability. There was no tendency for a greater number of loci to manifest instability in more advanced lesions. Neither anatomic location nor loss of heterozygosity at the p53 locus were associated with microsatellite instability by 2-way table analysis. These data support a role for defective DNA repair in the generation of a subset of both early and advanced ulcerative colitis-associated colorectal neoplastic lesions.
1 Supported by NIH Grant R01 DK47717 and the Crohn's and Colitis Foundation of America.
2 To whom requests for reprints should be addressed, at the University of Maryland N3W62, 22 South Greene Street, Baltimore, MD 21201.
Received 6/23/94. Accepted 8/ 3/94.
This article has been cited by other articles:
|
|
 |
|
  M. Svrcek, J. El-Bchiri, A. Chalastanis, E. Capel, S. Dumont, O. Buhard, C. Oliveira, R. Seruca, C. Bossard, J.-F. Mosnier, et al. Specific Clinical and Biological Features Characterize Inflammatory Bowel Disease Associated Colorectal Cancers Showing Microsatellite Instability J. Clin. Oncol., September 20, 2007; 25(27): 4231 - 4238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I.-Y. Chang, S.-H. Kim, H.-J. Cho, D. Y. Lee, M.-H. Kim, M.-H. Chung, and H. J. You Human AP endonuclease suppresses DNA mismatch repair activity leading to microsatellite instability Nucleic Acids Res., September 7, 2005; 33(16): 5073 - 5081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C R Boland, M G Luciani, C Gasche, and A Goel INFECTION, INFLAMMATION, AND GASTROINTESTINAL CANCER Gut, September 1, 2005; 54(9): 1321 - 1331. [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Potter, J. A. Murray, J. H. Donohue, L. J. Burgart, D. M. Nagorney, J. A. van Heerden, M. F. Plevak, A. R. Zinsmeister, and S. N. Thibodeau The Role of Defective Mismatch Repair in Small Bowel Adenocarcinoma in Celiac Disease Cancer Res., October 1, 2004; 64(19): 7073 - 7077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Svrcek, F Jourdan, N Sebbagh, A Couvelard, D Chatelain, N Mourra, S Olschwang, D Wendum, and J-F Flejou Immunohistochemical analysis of adenocarcinoma of the small intestine: a tissue microarray study J. Clin. Pathol., December 1, 2003; 56(12): 898 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Seril, J. Liao, G.-Y. Yang, and C. S. Yang Oxidative stress and ulcerative colitis-associated carcinogenesis: studies in humans and animal models Carcinogenesis, March 1, 2003; 24(3): 353 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Lee, D. K. Chang, A. Goel, C. R. Boland, W. Bugbee, D. L. Boyle, and G. S. Firestein Microsatellite Instability and Suppressed DNA Repair Enzyme Expression in Rheumatoid Arthritis J. Immunol., February 15, 2003; 170(4): 2214 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.K. Field and J.H. Youngson The Liverpool Lung Project: a molecular epidemiological study of early lung cancer detection Eur. Respir. J., August 1, 2002; 20(2): 464 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Chang, G. Marra, D. P. Chauhan, H. T. Ha, D. K. Chang, L. Ricciardiello, A. Randolph, J. M. Carethers, and C. R. Boland Oxidative stress inactivates the human DNA mismatch repair system Am J Physiol Cell Physiol, July 1, 2002; 283(1): C148 - C154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gasche, C. L. Chang, J. Rhees, A. Goel, and C. R. Boland Oxidative Stress Increases Frameshift Mutations in Human Colorectal Cancer Cells Cancer Res., October 1, 2001; 61(20): 7444 - 7448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Sohn, S. A. Shah, S. Reid, M. Choi, J. Carrier, M. Comiskey, C. Terhorst, and Y.-I. Kim Molecular Genetics of Ulcerative Colitis-associated Colon Cancer in the Interleukin 2- and {beta}2-Microglobulin-deficient Mouse Cancer Res., September 1, 2001; 61(18): 6912 - 6917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Ishitsuka, H Kashiwagi, and F Konishi Microsatellite instability in inflamed and neoplastic epithelium in ulcerative colitis J. Clin. Pathol., July 1, 2001; 54(7): 526 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sturlan, G. Oberhuber, B. G. Beinhauer, B. Tichy, S. Kappel, J. Wang, and M. A. Rogy Interleukin-10-deficient mice and inflammatory bowel disease associated cancer development Carcinogenesis, April 1, 2001; 22(4): 665 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Liloglou, P. Maloney, G. Xinarianos, M. Hulbert, M. J. Walshaw, J. R. Gosney, L. Turnbull, and J. K. Field Cancer-specific Genomic Instability in Bronchial Lavage: A Molecular Tool for Lung Cancer Detection Cancer Res., February 1, 2001; 61(4): 1624 - 1628. [Abstract] [Full Text]
|
|
|
|
|
|
A. S. Fleisher, M. Esteller, N. Harpaz, A. Leytin, A. Rashid, Y. Xu, J. Liang, O. C. Stine, J. Yin, T.-T. Zou, et al. Microsatellite Instability in Inflammatory Bowel Disease-associated Neoplastic Lesions Is Associated with Hypermethylation and Diminished Expression of the DNA Mismatch Repair Gene, hMLH1 Cancer Res., September 1, 2000; 60(17): 4864 - 4868. [Abstract] [Full Text]
|
|
|
|
|
|
S H ITZKOWITZ Microsatellite instability in colitis associated colorectal cancer Gut, March 1, 2000; 46(3): 304 - 305. [Full Text] [PDF]
|
|
|
|
|
|
L Cawkwell, F Sutherland, H Murgatroyd, P Jarvis, S Gray, D Cross, N Shepherd, D Day, and P Quirke Defective hMSH2/hMLH1 protein expression is seen infrequently in ulcerative colitis associated colorectal cancers Gut, March 1, 2000; 46(3): 367 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Walsh, M. Murphy, M. Silverman, R. Odze, D. Antonioli, H. Goldman, and M. Loda p27 Expression in Inflammatory Bowel Disease-Associated Neoplasia : Further Evidence of a Unique Molecular Pathogenesis Am. J. Pathol., November 1, 1999; 155(5): 1511 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Willenbucher, D. E. Aust, C. G. Chang, S. J. Zelman, L. D. Ferrell, D. H. Moore II, and F. M. Waldman Genomic Instability Is an Early Event during the Progression Pathway of Ulcerative-Colitis-Related Neoplasia Am. J. Pathol., June 1, 1999; 154(6): 1825 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Fleisher, M. Esteller, S. Wang, G. Tamura, H. Suzuki, J. Yin, T.-T. Zou, J. M. Abraham, D. Kong, K. N. Smolinski, et al. Hypermethylation of the hMLH1 Gene Promoter in Human Gastric Cancers with Microsatellite Instability Cancer Res., March 1, 1999; 59(5): 1090 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S-W Choi, Y-I Kim, J N Weitzel, and J B Mason Folate depletion impairs DNA excision repair in the colon of the rat Gut, July 1, 1998; 43(1): 93 - 99. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
