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Isolation and Identification of Fresh Tumor-derived Endothelial Cells from a Murine RIF-1 Fibrosarcoma¹

Department of Otolaryngology [R. A. M., M-J. C., C. S. J.], Pharmacology [P. D., C. S. J.], and Neuroanatomy and Cell Biology [S. C. W], Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and Department of Zoology, University of Wisconsin, Madison, Wisconsin 53706 [R. A.]

² To whom requests for reprints should be addressed, at University of Pittsburgh School of Medicine, Department of Otolaryngology, 203 Lothrop Street, Room 110, Pittsburgh, PA 15213.

Tumor vasculature is anatomically heterogeneous and distinct from the vasculature found in normal mature tissues. Examination of the differences between tumor and normal vasculature is critical to the future design of therapeutic modalities which either target tumor vasculature or potentially enable more efficient delivery of tumor cytotoxic agents. Such efforts to date have been hampered due to the inability to isolate live endothelial cells from solid tumors. We report here the isolation of fresh, noncultured endothelial cells from a C3H/HeJ RIF-1 murine fibrosarcoma through the use of fluorescence-activated cell sorting based on antibody staining for angiotensin-converting enzyme with further characterization by uptake and metabolism of acetylated low-density lipoprotein, factor VIII staining, and electron microscopy.

¹ This work was supported by Public Health Service Grants CA48077 and CA56756 from the National Cancer Institute, NIH, and by the Mary Hillman Jennings Foundation.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 9/30/93. Accepted 11/26/93.

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