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Messenger RNA Levels of Five Genes Located at Chromosome 11q13 in B-Cell Tumors with Chromosome Translocation t(11;14)(q13;q32)¹

Genetics Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104 [N. A., H. T., H. Sas., H. Sak., M. H., Y. O., T. S., M. T.]; Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113 [N. A., H. H., Y. Y.];; Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464 [M. S., R. U.], Japan

³ To whom requests for reprints should be addressed.

To identify genes activated by chromosome translocation t(11;14)–(q13;q32), mRNA levels of five genes (cyclin D1, EXP1, MB38, HST1, and INT2) at chromosome 11q13 were investigated. The cyclin D1 mRNA increased in BCL-1-rearranged B-cell tumor cell lines SP-49, NOP-2, FLAM-76, KMS-12-PE, and KMS-12-BM cells, while it was not detected in cell lines without the translocation, Raji, U266, and HEL cells. A significant amount of the MB38 mRNA was detected irrelevantly to the translocation in all of these cell lines. The mRNAs of EXP1, HST1, and INT2 were undetectable in these cells. The results suggested that the translocation activates cyclin D1 alone, while the mRNA levels of the other four genes are regulated independently of the translocation.

¹ This work was supported in part by a Grant-in-Aid for Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Heahh and Welfare of Japan; Grants-in-Aid from the Ministry of Health and Welfare and from the Ministry of Education, Science and Culture of Japan; the Bristol-Myers Squibb Foundation; and the Uehara Memorial Foundation.

² Recipient of a Research Resident Fellowship from the Foundation for Promotion of Cancer Research.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 11/30/93. Accepted 12/ 6/93.

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