| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Environmental Epidemiology Branch, National Cancer Institute, Bethesda, Maryland 20892 [N. P., R. T. F., P. K. S., R. N. H.], and Microbiological Associates, Inc., Rockville, Maryland 20850 [V. A. L.]
The relationship of serum hormones to cancer risk has recently been pursued in epidemiological studies, but few have reported on the reproducibility of laboratory findings. Prior to conducting a study of endogenous hormones and endometrial cancer, we evaluated the reproducibility of measurements for several hormones (estrone, estradiol, free estradiol, albumin-bound estradiol, and androstenedione) and sex hormone-binding globulin. We obtained a single unit of blood from each of six women and prepared aliquots of serum for repeated testing. Three laboratories analyzed multiple samples on consecutive working days from which estimates of intraassay and interassay measurement variability were obtained. For estrone and estradiol, a log transformation of the data produced distributions which were nearly normal and permitted the use of parametric statistical tests. In general, we found measurements for most hormones varied considerably between assays. Moreover, differences were observed in the absolute values of sex hormone-binding globulin and of the hormones, particularly for estrone and estradiol, from one laboratory to the next. Our findings suggest that variability of current laboratory procedures may hamper efforts to study the association between disease and endogenous hormones in epidemiological studies. In addition, validation of hormone assays is essential in order to assure standardized results and enable comparisons of data across studies.
1 To whom requests for reprints should be addressed, at Environmental Epidemiology Branch, National Cancer Institute, Executive Plaza North, Suite 443, Bethesda, MD 20892.
Received 5/25/94. Accepted 8/15/94.
This article has been cited by other articles:
|
|
 |
|
  N. E Allen, T. J Key, L. Dossus, S. Rinaldi, A. Cust, A. Lukanova, P. H Peeters, N C. Onland-Moret, P. H Lahmann, F. Berrino, et al. Endogenous sex hormones and endometrial cancer risk in women in the European Prospective Investigation into Cancer and Nutrition (EPIC) Endocr. Relat. Cancer, June 1, 2008; 15(2): 485 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Hsing, F. Z. Stanczyk, A. Belanger, P. Schroeder, L. Chang, R. T. Falk, and T. R. Fears Reproducibility of Serum Sex Steroid Assays in Men by RIA and Mass Spectrometry Cancer Epidemiol. Biomarkers Prev., May 1, 2007; 16(5): 1004 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. Goderie-Plomp, M. van der Klift, W. de Ronde, A. Hofman, F. H. de Jong, and H. A. P. Pols Endogenous Sex Hormones, Sex Hormone-Binding Globulin, and the Risk of Incident Vertebral Fractures in Elderly Men and Women: The Rotterdam Study J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3261 - 3269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Taieb, C. Benattar, R. Diop, A. S. Birr, and A. Lindenbaum Use of the Architect-i2000 Estradiol Immunoassay during in Vitro Fertilization Clin. Chem., January 1, 2003; 49(1): 183 - 186. [Full Text] [PDF]
|
|
|
|
|
|
J. Taieb, C. Benattar, A. S. Birr, and A. Lindenbaum Limitations of Steroid Determination by Direct Immunoassay Clin. Chem., March 1, 2002; 48(3): 583 - 585. [Full Text] [PDF]
|
|
|
|
|
|
S. Rinaldi, H. Dechaud, C. Biessy, V. Morin-Raverot, P. Toniolo, A. Zeleniuch-Jacquotte, A. Akhmedkhanov, R. E. Shore, G. Secreto, A. Ciampi, et al. Reliability and Validity of Commercially Available, Direct Radioimmunoassays for Measurement of Blood Androgens and Estrogens in Postmenopausal Women Cancer Epidemiol. Biomarkers Prev., July 1, 2001; 10(7): 757 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Garcia-Closas, S. E. Hankinson, S.-m. Ho, D. C. Malins, N. L. Polissar, S. N. Schaefer, Y. Su, and M. A. Vinson Chapter 9: Factors Critical to the Design and Execution of Epidemiologic Studies and Description of an Innovative Technology to Follow the Progression From Normal to Cancer Tissue J Natl Cancer Inst Monographs, July 1, 2000; 2000(27): 147 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Barrett-Connor, J. E. Mueller, D. G. von Mühlen, G. A. Laughlin, D. L. Schneider, and D. J. Sartoris Low Levels of Estradiol Are Associated with Vertebral Fractures in Older Men, But Not Women: The Rancho Bernardo Study J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 219 - 223. [Abstract] [Full Text]
|
|
|
|
|
|
R. T. Falk, M. H. Gail, T. R. Fears, S. C. Rossi, F. Stanczyk, H. Adlercreutz, P. Kiura, K. Wahala, J. L. Donaldson, J. B. Vaught, et al. Reproducibility and Validity of Radioimmunoassays for Urinary Hormones and Metabolites in Pre- and Postmenopausal Women Cancer Epidemiol. Biomarkers Prev., June 1, 1999; 8(6): 567 - 577. [Abstract] [Full Text]
|
|
|
|
|
|
A. Shibata, M. M. Lee, and P. B. Meyer Laboratory Assay Reproducibility of Serum Estrogens in Umbilical Cord Blood Samples Cancer Epidemiol. Biomarkers Prev., February 1, 1999; 8(2): 147 - 151. [Abstract] [Full Text]
|
|
|
|
|
|
A. Levesque, M. Letellier, P. W. Dillon, and A. Grant Analytical performance of Bayer Immuno 1TM estradiol and progesterone assays Clin. Chem., September 1, 1997; 43(9): 1601 - 1609. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
