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Molecular Cloning of the B-CAM Cell Surface Glycoprotein of Epithelial Cancers: A Novel Member of the Immunoglobulin Superfamily

Obstetrics and Gynaecology, University of Southampton, Princess Anne Hospital, Coxford Road, Southampton, SO16 5YA, United Kingdom [I. G. C.]; Cytogenetics [G. S.] and Human Immunogenetics [J. T.] Laboratories, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom; and Ludwig Institute for Cancer Research [P. G-C., W. J. R.], Department of Pathology [P. G-C.], and Immunology Programme [W. J. R.], Memorial Sloan-Kettering Cancer Center, New York, New York 10021

The human F8/G253 antigen, B-CAM, is a cell surface glycoprotein that is expressed with restricted distribution pattern in normal fetal and adult tissues, and is up-regulated following malignant transformation in some cell types. We have isolated a complementary DNA for B-CAM using an expression cloning technique. The complementary DNA (EMBL accession number X80026) encodes a 588-amino acid protein which is a novel member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 immunoglobulin domain structure. This structure has been described previously for the human MUC18 melanoma antigen (31% amino acid identity) and chicken and rat versions of a neural adhesion molecule referred to as SC1/DM-GRASP/BEN or KG-CAM, respectively (26% amino acid identity). This homology is suggestive of a role for B-CAM in cell-cell or cell-matrix adhesion. The gene for B-CAM has been mapped by fluorescence in situ hybridization to chromosome 19q13.2–13.3.

¹ To whom requests for reprints should be addressed.

Received 10/ 3/94. Accepted 10/14/94.

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