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Mutation Rate of the CDKN2 Gene in Malignant Gliomas¹

Istituto Nazionale Neurologico "C. Besta," Divisione di Biochimica e Genetica, via Celoria 11, 20133 Milan, Italy

The CDKN2 gene encodes p16, a protein controlling the cell cycle. CDKN2 is deleted in a relevant number of tumor cell lines, but results of the studies in primary tumors are contradictory. We have investigated by using quantitative polymerase chain reaction and single-strand conformation polymorphism analysis the structure of exon 2 of CDKN2 in 32 malignant gliomas. In 11 tumors the amount of amplified material was 21% of that of controls and in 8 tumors it was 42.3%, suggesting the presence of homozygous and hemizygous deletions of the CDKN2 gene, respectively. However, no abnormality could be detected by single-strand conformation polymorphism analysis. The data confirm in primary gliomas that homozygous deletions are a mechanism of CDKN2 inactivation and suggest that another gene in the vicinity could be targeted by mutations.

¹ This work has been partially supported by a grant from the Associazione Italiana per la Ricerca sul Cancro [G. F.].

² To whom requests for reprints should be addressed.

Received 8/18/94. Accepted 11/ 1/94.

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