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Characterization of a Rana catesbeiana Lectin-resistant Mutant of Leukemia P388 Cells¹

Cancer Research Institute and Departments of Pharmaceutics and Radiopharmacy, Tohoku College of Pharmaceutical Sciences, Sendai 981, Japan [K. N., K. O., Y T., S. F., Y. O.,H. T., R. M., M. H., N. H., K-i. S., H. K., Y. T., S. T.], The Biomembrane Institute and University of Washington, Seattle, Washington 98119 [S-i. H.]

² To whom requests for reprints should be addressed at Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, 4-4-1 Komatsushima, Aobaku, Sendai 981, Japan.

Sialic acid-binding lectin (SBL-C) from Rana catesbeiana eggs inhibits the growth of tumor cells such as P388 and L1210 leukemia cells (K. Nitta et al., Cancer Res., 54: 920–927, 1994). Here we report the establishment of an SBL-resistant P388 variant cell line, RC-150. Both P388 and RC-150 cells were agglutinated by SBL-C; however, growth of RC-150 cells was unaffected by SBL-C. Cytoplasmic free Ca²⁺ concentration and transglutaminase activity of RC-150 cells were 0.5 (110 nM) and 3 times (0.62 nmol/mg/min) as high as those of P388 cells, respectively. Microvilli and microplicae were observed on the surface of P388 cells by scanning electron microscopy but were rarely seen on RC-150 cells. Dansylcadaverine-labeled SBL-C bound to both P388 and RC-150 cells. Binding of SBL-C to these tumor cells appears to be mediated by two species of wheat germ agglutinin-stained cell membrane sialoglycoproteins. Labeled SBL-C entered P388 but not RC-150 cells, suggesting that internalized SBL-C acts as an inhibitor of cell proliferation.

¹ This study was supported by The Science Research Promotion Fund from the Japan Private School Promotion Foundation.

³ Present address: Department of Diagnostic Reagent Development, Diagnostic Division, Otsuka Pharmaceutical Co., Ltd., Tokushima 771-01, Japan.

⁴ Present address: Radiation Chemistry, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422, Japan.

⁵ Present address: Health Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422, Japan.

⁶ Deceased.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 8/ 4/93. Accepted 12/16/93.