| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy [N. S. P., B. R., G. N. R.]; Department of Pathology, Ospedale di Circolo di Varese, 21100 Varese, Italy [F. S., M. B.]; Department of Pathology, Istituto di Ricovero e Cura a Carattere Scientifico-Ospedale S. Raffaele, Milano, Italy [B. E. L.]; Department of Human Pathology, University of Pavia and Istituto di Ricovero e Cura a Carattere Scientifico-Ospedale S. Matteo, 27100 Pavia, Italy [E. S.]
We studied K-ras and p53 gene mutations in a panel of 57 primary pancreatic cancers including ductal and nonductal tumors. DNAs were obtained from formalin-fixed, paraffin-embedded material. Target sequences were amplified by polymerase chain reaction and analyzed by denaturing gradient gel electrophoresis and sequencing. Both K-ras and p53 genes were frequently mutated in ductal cancers (25 of 35, 71.4%; 18 of 35, 51.4%, respectively). K-ras mutations were confined to the second position of codon 12 where base transitions and transversions were equally observed. p53 changes were mainly missense mutations. Transitions and transversions were found equally with a prevalence of G:C
A:T changes among transitions. No gene alterations were present in the 6 exocrine nonductal tumors and (with one exception) in the 12 endocrine tumors analyzed. Our results indicate that mutated K-ras and p53 genes can cooperate in the establishment of ductal pancreatic cancers, whereas other genetic events have to be present in nonductal tumors. Moreover, K-ras alterations may represent an early event in ductal tumorigenesis, as suggested both by the high gene mutation frequency and by the presence of mutations in low-grade tumors. On the contrary, p53 gene changes seem to represent an event required for the malignancy progression of ductal tumors from lower to higher grades.
1 This work was supported by a grant from the Ministero Università e Ricerca Scientifica to G.N.R.
2 To whom requests for reprints should be addressed, at Dip. di Genetica e Microbiologia, Via Abbiategrasso 207, 27100 Pavia, Italy.
Received 9/ 7/93. Accepted 1/14/94.
This article has been cited by other articles:
|
|
 |
|
  C Brockschmidt, H Hirner, N Huber, T Eismann, A Hillenbrand, G Giamas, B Radunsky, O Ammerpohl, B Bohm, D Henne-Bruns, et al. Anti-apoptotic and growth-stimulatory functions of CK1 delta and epsilon in ductal adenocarcinoma of the pancreas are inhibited by IC261 in vitro and in vivo Gut, June 1, 2008; 57(6): 799 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Trapasso, S. Yendamuri, K. R. Dumon, R. Iuliano, R. Cesari, B. Feig, R. Seto, L. Infante, H. Ishii, A. Vecchione, et al. Restoration of receptor-type protein tyrosine phosphatase {eta} function inhibits human pancreatic carcinoma cell growth in vitro and in vivo Carcinogenesis, November 1, 2004; 25(11): 2107 - 2114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Rodicker and B. M. Putzer p73 Is Effective in p53-null Pancreatic Cancer Cells Resistant to Wild-type TP53 Gene Replacement Cancer Res., June 1, 2003; 63(11): 2737 - 2741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Ashley and G. Y. Lauwers Case 37-2002 - A 69-Year-Old Man with Painful Cutaneous Nodules, Elevated Lipase Levels, and Abnormal Results on Abdominal Scanning N. Engl. J. Med., November 28, 2002; 347(22): 1783 - 1791. [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Abraham, D. S. Klimstra, R. E. Wilentz, C. J. Yeo, K. Conlon, M. Brennan, J. L. Cameron, T.-T. Wu, and R. H. Hruban Solid-Pseudopapillary Tumors of the Pancreas Are Genetically Distinct from Pancreatic Ductal Adenocarcinomas and Almost Always Harbor {beta}-catenin Mutations Am. J. Pathol., April 1, 2002; 160(4): 1361 - 1369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Abraham, T.-T. Wu, R. H. Hruban, J.-H. Lee, C. J. Yeo, K. Conlon, M. Brennan, J. L. Cameron, and D. S. Klimstra Genetic and Immunohistochemical Analysis of Pancreatic Acinar Cell Carcinoma : Frequent Allelic Loss on Chromosome 11p and Alterations in the APC/{beta}-Catenin Pathway Am. J. Pathol., March 1, 2002; 160(3): 953 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rodicker, T. Stiewe, S. Zimmermann, and B. M. Putzer Therapeutic Efficacy of E2F1 in Pancreatic Cancer Correlates with TP73 Induction Cancer Res., October 1, 2001; 61(19): 7052 - 7055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. W. Ng, M.-S. Tsao, T. Nicklee, and D. W. Hedley Wortmannin Inhibits PKB/Akt Phosphorylation and Promotes Gemcitabine Antitumor Activity in Orthotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice Clin. Cancer Res., October 1, 2001; 7(10): 3269 - 3275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rowland-Goldsmith, H. Maruyama, T. Kusama, S. Ralli, and M. Korc Soluble Type II Transforming Growth Factor-{beta} (TGF-{beta}) Receptor Inhibits TGF-{beta} Signaling in COLO-357 Pancreatic Cancer Cells in Vitro and Attenuates Tumor Formation Clin. Cancer Res., September 1, 2001; 7(9): 2931 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Rigaud, E. Missiaglia, P. S. Moore, G. Zamboni, M. Falconi, G. Talamini, A. Pesci, A. Baron, D. Lissandrini, G. Rindi, et al. High Resolution Allelotype of Nonfunctional Pancreatic Endocrine Tumors: Identification of Two Molecular Subgroups with Clinical Implications Cancer Res., January 1, 2001; 61(1): 285 - 292. [Abstract] [Full Text]
|
|
|
|
|
|
S. S. W. Ng, M.-S. Tsao, S. Chow, and D. W. Hedley Inhibition of Phosphatidylinositide 3-Kinase Enhances Gemcitabine-induced Apoptosis in Human Pancreatic Cancer Cells Cancer Res., October 1, 2000; 60(19): 5451 - 5455. [Abstract] [Full Text]
|
|
|
|
|
|
H. J. Wanebo and R. K. Yantiss Case 26-2000- A 47-Year-Old Man with Recurrent Pancreatitis and a Cystic Pancreatic Mass N. Engl. J. Med., August 24, 2000; 343(8): 563 - 570. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, D. L. Weddle, P. E. Thomas, B. Zheng, A. Castonguay, H. M. Schuller, and M. S. Miller Low Levels of Expression of Cytochromes P-450 in Normal and Cancerous Fetal Pancreatic Tissues of Hamsters Treated with NNK and/or Ethanol Toxicol. Sci., August 1, 2000; 56(2): 313 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Heinmoller, W. Dietmaier, H. Zirngibl, P. Heinmoller, W. Scaringe, K.-W. Jauch, F. Hofstadter, and J. Ruschoff Molecular Analysis of Microdissected Tumors and Preneoplastic Intraductal Lesions in Pancreatic Carcinoma Am. J. Pathol., July 1, 2000; 157(1): 83 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Bruns, C. C. Solorzano, M. T. Harbison, S. Ozawa, R. Tsan, D. Fan, J. Abbruzzese, P. Traxler, E. Buchdunger, R. Radinsky, et al. Blockade of the Epidermal Growth Factor Receptor Signaling by a Novel Tyrosine Kinase Inhibitor Leads to Apoptosis of Endothelial Cells and Therapy of Human Pancreatic Carcinoma Cancer Res., June 1, 2000; 60(11): 2926 - 2935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Hruban, R. E. Wilentz, and S. E. Kern Genetic Progression in the Pancreatic Ducts Am. J. Pathol., June 1, 2000; 156(6): 1821 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dong, Y. Nio, K. Tamura, M.-M. Song, K.-J. Guo, R.-X. Guo, and Y.-T. Dong Ki-ras Point Mutation and p53 Expression in Human Pancreatic Cancer: A Comparative Study among Chinese, Japanese, and Western Patients Cancer Epidemiol. Biomarkers Prev., March 1, 2000; 9(3): 279 - 284. [Abstract] [Full Text]
|
|
|
|
|
|
E. Hidaka, A. Yanagisawa, M. Seki, K. Takano, T. Setoguchi, and Y. Kato High Frequency of K-ras Mutations in Biliary Duct Carcinomas of Cases with a Long Common Channel in the Papilla of Vater Cancer Res., February 1, 2000; 60(3): 522 - 524. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kuniyasu, L. M. Ellis, D. B. Evans, J. L. Abbruzzese, C. J. Fenoglio, C. D. Bucana, K. R. Cleary, E. Tahara, and I. J. Fidler Relative Expression of E-Cadherin and Type IV Collagenase Genes Predicts Disease Outcome in Patients with Resectable Pancreatic Carcinoma Clin. Cancer Res., January 1, 1999; 5(1): 25 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Berndt, K. Haubold, F. Wenger, B. Brux, J. Muller, P. Bendzko, T. Hillebrand, E. Kottgen, and J. Zanow K-ras mutations in stools and tissue samples from patients with malignant and nonmalignant pancreatic diseases Clin. Chem., October 1, 1998; 44(10): 2103 - 2107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Liu, T. Furukawa, M. Kobari, and M.-S. Tsao Comparative Phenotypic Studies of Duct Epithelial Cell Lines Derived from Normal Human Pancreas and Pancreatic Carcinoma Am. J. Pathol., July 1, 1998; 153(1): 263 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Pitot The Progression of Neoplasia, Cell Replication, and Electromagnetic Fields International Journal of Toxicology, January 1, 1998; 17(3_suppl): 59 - 108. [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
