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A Monoclonal Antibody Inhibits Adhesion to Fibronectin and Vitronectin of a Colon Carcinoma Cell Line and Recognizes the Integrins _vß₃, _vß₅, and _vß₆¹

Institut de Chimie Biologique, ER CNRS 079, Université d'Aix-Marseille I, 3 place Victor Hugo 13331 Marseille Cedex 3 [M. L., C. R., J. P., J. M.]; Institut de Biologie et Chimie des Protéines, UPR CNRS 412, 7 passage du Vercors, 69007 Lyon, France [J-C. L.]; and Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037 [R. T., V. Q.]

Using whole viable human colon carcinoma HT29 cells as immunogen, we produced a monoclonal antibody (mAb) termed 69-6-5. The antibody was functionally selected on its anti-cell-spreading activity. By immunoprecipitation of surface radiolabeled cell lysates from HT29-D4 cells (an HT29 cell clone), mAb 69-6-5 recognized a molecular complex resembling integrin heterodimers. Sequential immunodepletions with mAb to the integrin _v subunit demonstrated that this complex was composed of _v-containing integrins. Accordingly, mAb 69-6-5 reacted with integrin _vß₃ immunopurified from melanoma cells and integrins _vß₅ and _vß₆ immunopurified from pancreatic carcinoma cells. In cell adhesion assays, the 69-6-5 mAb was able to inhibit strongly in a dose-dependent manner arginine-glycine-aspartic acid-mediated adhesion of HT29-D4 cells to vitronectin, fibronectin, or ProNectin F but not to laminin or collagen. Immunoprecipitations with ß chain-specific antisera indicated that these cells express integrins _vß₅ (receptor for vitronectin) and _vß₆ (receptor for fibronectin) but neither _vß₁ nor _vß₃.

In summary, these results indicated that mAb 69-6-5 reacts with several _v integrins and that it can effectively interfere with the adhesive functions of at least _vß₅ and _vß₆, which represent the major receptors on HT29-D4 cells responsible for their adhesion on vitronectin and fibronectin.

¹ This work was supported by Institut National de la Santé et de la Recherche Médicale Grant CRE 920 205, the Fédération Nationale des Centres de Lutte Contre le Cancer, and the Association de Recherche sur le Cancer.

² To whom requests for reprints should be addressed.

³ These two authors contributed equally to the completion of the biochemical part of this work.

Received 5/21/93. Accepted 2/18/94.

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