This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cesano, A. Articles by Santoli, D. Search for Related Content PubMed PubMed Citation Articles by Cesano, A. Articles by Santoli, D.

Treatment of Experimental Glioblastoma with a Human Major Histocompatibility Complex Nonrestricted Cytotoxic T Cell Line¹

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

This study investigates a new approach to adoptive therapy of glioblastoma using as antitumor effector a potent major histocompatibility complex nonrestricted killer clone (TALL-104) established from a patient with acute T-lymphoblastic leukemia. The human glioblastoma cell line U-87 MG could be successfully engrafted in mice with severe combined immunodeficiency using the i.p., intracerebral, and s.c. routes. The latter model was elected to evaluate therapy based on its high reproducibility. Tumor growth in mice engrafted s.c. was proportionally associated with splenomegaly and leukocytosis. Multiple transfers of lethally irradiated (non-proliferating) TALL-104 cells at the tumor site resulted in about 50–70% inhibition of tumor growth as compared to untreated mice, with concomitant reduction of splenomegaly and leukocytosis. The antitumor effects were inversely proportional to the size of the tumor at initiation of therapy, 90–100% inhibition occurring in severe combined immunodeficiency mice treated from the day of U-87 MG challenge. Neither splenomegaly nor leukocytosis developed in animals in which tumor growth was completely blocked. Stimulation of TALL-104 cells with either interleukin 2 or interleukin 12 prior to irradiation and adoptive transfer increased the antitumor efficacy of the killer cells to about the same extent. The potential usefulness of irradiated TALL-104 cells in adjuvant therapy against glioblastomas and other well-localized tumors is discussed.

¹ This work was supported by American Cancer Society Grants CH-527 and DH-107A, NIH Grant CA-10815, and the Taxin Memorial Fund. A. C. is partially supported by the José Carreras International Leukemia Foundation.

² To whom requests for reprints should be addressed, at The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104-4268.

Received 8/ 3/94. Accepted 10/28/94.

This article has been cited by other articles:

				C. Brando, S. Mukhopadhyay, E. Kovacs, R. Medina, P. Patel, T. L. Catina, K. S. Campbell, and D. Santoli Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104 J. Leukoc. Biol., August 1, 2005; 78(2): 359 - 371. [Abstract] [Full Text] [PDF]

				G. G. Gomez, S. B. Read, L. E. Gerschenson, D. Santoli, A. Zweifach, and C. A. Kruse Interactions of the allogeneic effector leukemic T cell line, TALL-104, with human malignant brain tumors Neuro-oncol, April 1, 2004; 6(2): 83 - 95. [Abstract] [PDF]

				C. A. Kruse, S. Visonneau, B. K. Kleinschmidt-DeMasters, C. J. Gup, G. G. Gomez, D. B. Paul, and D. Santoli The Human Leukemic T-Cell Line, TALL-104, Is Cytotoxic to Human Malignant Brain Tumors and Traffics through Brain Tissue: Implications for Local Adoptive Immunotherapy Cancer Res., October 1, 2000; 60(20): 5731 - 5739. [Abstract] [Full Text]

				S. Visonneau, A. Cesano, D. L. Porter, S. L. Luger, L. Schuchter, M. Kamoun, M. H. Torosian, K. Duffy, C. Sickles, E. A. Stadtmauer, et al. Phase I Trial of TALL-104 Cells in Patients with Refractory Metastatic Breast Cancer Clin. Cancer Res., May 1, 2000; 6(5): 1744 - 1754. [Abstract] [Full Text]

				B. Geoerger, C.-B. Tang, A. Cesano, S. Visonneau, S. Marwaha, K. D. Judy, L. N. Sutton, D. Santoli, and P. C. Phillips Antitumor activity of a human cytotoxic T-cell line (TALL-104) in brain tumor xenografts Neuro-oncol, April 1, 2000; 2(2): 103 - 113. [Abstract] [PDF]

				S. Visonneau, A. Cesano, K. A. Jeglum, and D. Santoli Adjuvant Treatment of Canine Osteosarcoma with the Human Cytotoxic T-Cell Line TALL-104 Clin. Cancer Res., July 1, 1999; 5(7): 1868 - 1875. [Abstract] [Full Text] [PDF]