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Identification of a Sister Gene to P-Glycoprotein¹

Department of Medical Biophysics, Ontario Cancer Institute, Toronto, Ontario M4X 1K9, Canada [S. C., R. L. Y., V. L.], and Institute of Parasitology, McGill University, Ste-Anne de Bellevue, Quebec H9X 3V9, Canada [E. G.]

The P-glycoproteins (Pgps) are a small family of transport proteins associated with the multidrug resistance phenotype of cell lines selected for growth in cytotoxic drugs. Utilizing low stringency screening, we have identified a novel gene closely related to the Pgps expressed in the pig and other mammalian liver which we have called Sister of P-glycoprotein (spgp). Sequence of this gene shows it to be a member of the ATP-binding cassette family of transporters and the gene most closely related to Pgp identified to date. The function of spgp is not known, but it can be recognized by at least one Pgp mAb, C219. This cross-reactivity has implications for expression studies in tissues and tumors utilizing this and other Pgp antibodies.

¹ S. C. is a recipient of a Steve Fonyo Studentship from the National Cancer Institute of Canada.

² To whom requests for reprints should be addressed, at Ontario Cancer Institute, Department of Medical Biophysics, 500 Sherbourne Street, Toronto Ontario M4X 1K9, Canada.

Received 2/13/95. Accepted 4/ 5/95.

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