This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beltinger, C. P. Articles by Brodeur, G. M. Search for Related Content PubMed PubMed Citation Articles by Beltinger, C. P. Articles by Brodeur, G. M.

No CDKN2 Mutations in Neuroblastomas¹

Division of Oncology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104-9786

Mutations of CDKN2 have been found recently in melanoma and many other tumor types. Neuroblastoma shares with melanoma a neuroectodermal origin and a high incidence of deletions of the short arm of chromosome 1. Therefore, we analyzed 18 primary neuroblastomas and 9 tumor-derived cell lines for mutations in CDKN2. We used PCR-single-strand conformation polymorphism to examine exons 1 and 2 of the CDKN2 gene for mutations, but none were detected. Furthermore, no homozygous deletions were detected and there was no loss of heterozygosity at the closely linked IFNA locus. We conclude that disruption of the CDKN2 gene is not required for malignant transformation of human neuroblastomas.

¹ This work was supported by Grant CA 39771 (G. M. B.).

² To whom requests for reprints should be addressed, at Division of Oncology, Room 9000, Children's Hospital of Philadelphia, 324 South 34th Street, Philadelphia, PA 19104-9786.

Received 3/ 9/95. Accepted 4/ 5/95.

This article has been cited by other articles:

				Q. Wang, G. Hii, S. Shusterman, Y. Mosse, C. L. Winter, C. Guo, H. Zhao, E. Rappaport, M. D. Hogarty, and J. M. Maris ID2 Expression Is not Associated with MYCN Amplification or Expression in Human Neuroblastomas Cancer Res., April 1, 2003; 63(7): 1631 - 1635. [Abstract] [Full Text] [PDF]

				M. Yashiro, J. M. Carethers, L. Laghi, K. Saito, P. Slezak, E. Jaramillo, C. Rubio, K. Koizumi, K. Hirakawa, and C. R. Boland Genetic Pathways in the Evolution of Morphologically Distinct Colorectal Neoplasms Cancer Res., March 1, 2001; 61(6): 2676 - 2683. [Abstract] [Full Text]

				P. M. Thompson, J. M. Maris, M. D. Hogarty, R. C. Seeger, C. P. Reynolds, G. M. Brodeur, and P. S. White Homozygous Deletion of CDKN2A (p16INK4a/p14ARF) but not within 1p36 or at Other Tumor Suppressor Loci in Neuroblastoma Cancer Res., January 1, 2001; 61(2): 679 - 686. [Abstract] [Full Text]

				J. M. Maris and K. K. Matthay Molecular Biology of Neuroblastoma J. Clin. Oncol., July 1, 1999; 17(7): 2264 - 2264. [Abstract] [Full Text] [PDF]

				S E Coupland, N Bechrakis, A Schüler, I Anagnostopoulos, M Hummel, N Bornfeld, and H Stein Expression patterns of cyclin D1 and related proteins regulating G1-S phase transition in uveal melanoma and retinoblastoma Br J Ophthalmol, August 1, 1998; 82(8): 961 - 970. [Abstract] [Full Text]