| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, Maryland 20892
A novel immunoglobulin-type protein expressed in blood vessels has been identified. The cDNA for AAMP (angio-associated, migratory cell protein) was first isolated from a human melanoma cell line during a search for motility-associated cell surface proteins. Upon analysis of the tissue distribution of AAMP, it was found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The sequence of AAMP predicts a protein (Mr 49,000) with distant identity (25%) to known proteins. It contains immunoglobulin-like domains [one with multiple homologies to deleted in colon carcinoma (DCC) protein], the WD40 repeat motif, and a heparin-binding consensus sequence. A 1.6-kilobase mRNA transcript of AAMP is detected in tissue culture cell lines and tissues. Affinity-purified polyclonal antibodies, anti-recombinant AAMP, and anti-peptide 189 (AAMP derived) recognize a Mr 52,000 protein in human tissue and cellular extracts. The protein size is in keeping with the mRNA and predicted sequence. The AAMP-derived peptide, P189, contains a heparin-binding domain (dissociation constant, 14 pmol) and mediates heparin-sensitive cell adhesion. The shared expression of AAMP in endothelial cells, trophoblasts, and tumor cells implies a common function in migrating cells.
1 To whom requests for reprints should be addressed, at Laboratory of Pathology, Bldg. 10, Rm. 2A-33, National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD 20892.
Received 9/26/94. Accepted 3/ 2/95.
This article has been cited by other articles:
|
|
 |
|
  F. Vogt, A. Zernecke, M. Beckner, N. Krott, A.-K. Bosserhoff, R. Hoffmann, M. A.M.J. Zandvoort, T. Jahnke, M. Kelm, C. Weber, et al. Blockade of Angio-Associated Migratory Cell Protein Inhibits Smooth Muscle Cell Migration and Neointima Formation in Accelerated Atherosclerosis J. Am. Coll. Cardiol., July 22, 2008; 52(4): 302 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Holvoet and P. Sinnaeve Angio-Associated Migratory Cell Protein and Smooth Muscle Cell Migration in Development of Restenosis and Atherosclerosis J. Am. Coll. Cardiol., July 22, 2008; 52(4): 312 - 314. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Adeyinka, E. Emberley, Y. Niu, L. Snell, L. C. Murphy, H. Sowter, C. C. Wykoff, A. L. Harris, and P. H. Watson Analysis of Gene Expression in Ductal Carcinoma in Situ of the Breast Clin. Cancer Res., December 1, 2002; 8(12): 3788 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.L. Stanton and D.P.L. Green A set of 840 mouse oocyte genes with well-matched human homologues Mol. Hum. Reprod., June 1, 2001; 7(6): 521 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ohene-Abuakwa and M. Pignatelli Adhesion Molecules as Diagnostic Tools in Tumor Pathology International Journal of Surgical Pathology, July 1, 2000; 8(3): 191 - 200. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Garlanda and E. Dejana Heterogeneity of Endothelial Cells : Specific Markers Arterioscler Thromb Vasc Biol, July 1, 1997; 17(7): 1193 - 1202. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Mendoza-Alvarez and R. Alvarez-Gonzalez Regulation of p53 Sequence-specific DNA-binding by Covalent Poly(ADP-ribosyl)ation J. Biol. Chem., September 21, 2001; 276(39): 36425 - 36430. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
