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Induction of Cyclobutane Pyrimidine Dimers, Pyrimidine(6-4)pyrimidone Photoproducts, and Dewar Valence Isomers by Natural Sunlight in Normal Human Mononuclear Cells¹

MRC Cell Mutation Unit, Sussex University, Falmer, Brighton, BN1 9RR, United Kingdom [P. H. C., C. F. A., M. H. L. G.]; TNO Nutrition and Food Research Institute, P.O. Box 5815.2280 HV, Rijswijk, the Netherlands [L. R.]; Nara Medical University, RI Centre, Kashihara, Nara 634, Japan [T. M.]; and Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa 920, Japan [O. N.]

Immunocytochemistry was used for the direct measurement of cyclobutane pyrimidine dimers, (6-4) photoproducts, and Dewar isomers in normal human mononuclear cells following irradiation by natural sunlight or by a FS20 broad spectrum UVB sunlamp. The induction of each type of photoproduct was detected following 30–60 min sunlight exposure or with FS20 fluences as low as 50–100 Jm^-2. With increasing FS20 fluences, there was a dose-dependent increase in the binding of pyrimidine dimer, (6-4) photoproduct, and Dewar isomer-specific monoclonal antibodies. The relative ratio of Dewar isomer to (6-4) photoproduct antibody binding sites was much higher following exposure to natural sunlight than to broad spectrum UVB. With the (6-4) monoclonal antibody, a small increase in binding sites was evident after a 1-h exposure to natural sunlight. This remained relatively constant with further exposure. These results are consistent with the hypothesis that, following irradiation with natural sunlight, the majority of (6-4) photoproducts are converted into Dewar valence isomers.

¹ Additional support was provided by the CEC program EV5VCT91-0034.

² To whom requests for reprints should be addressed.

Received 3/ 2/95. Accepted 4/20/95.

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