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[Cancer Research 55, 2487-2489, June 15, 1995]
© 1995 American Association for Cancer Research
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Effects of Estrogen on Apoptotic Pathways in Human Breast Cancer Cell Line MCF-7

Thomas T. Y. Wang1 and James M. Phang

Laboratory of Nutritional and Molecular Regulation, National Cancer Institute-Frederick Cancer Research and Development Center, NIH, Frederick, Maryland 21702-1201

We utilized a reverse transcription-PCR method to examine the effect of estrogen on the expression of mRNA for Bcl-2 and Bax, two modulatory proteins in the apoptotic pathway, in human breast cancer cell line MCF-7. We found that the bcl-2 mRNA levels in the cells exposed to 17ß-estradiol were higher than those of control cells. Although the relative bax mRNA levels remained unchanged, the changes in bcl-2 mRNA level occurred in a time- and concentration-dependent fashion. In addition, pretreatment with 17ß-estradiol protected MCF-7 cells from apoptosis. Our study provides evidence that responses of breast epithelial cells toward a steroid sex hormone involve regulation of the apoptotic pathway.

1 To whom requests for reprints should be addressed, at Laboratory of Nutritional and Molecular Regulation, Building 434/2, National Cancer Institute-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201.

Received 3/20/95. Accepted 5/ 5/95.




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