This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Herbst, R. A. Articles by Arden, K. C. Search for Related Content PubMed PubMed Citation Articles by Herbst, R. A. Articles by Arden, K. C.

A Defined Region of Loss of Heterozygosity at 11q23 in Cutaneous Malignant Melanoma¹

Ludwig Institute for Cancer Research [R. A. H., A. L., W. K. C., G. M. H., K. C. A.], Center for Molecular Genetics [W. K. C.], and Department of Medicine [W. K. C., K. C. A.], University of California at San Diego, La Jolla, California, 92093-0660, and the Department of Dermatology, Hannover Medical School, D 30449 Hannover, Germany [R. A. H., J. W.]

Karyotypic and molecular data indicate that genetic alterations of the long arm of chromosome 11 (11q) may be involved in malignant melanoma. To test this we analyzed 5 polymorphic microsatellite repeats on 11q using a PCR-based assay for loss of heterozygosity in normal and tumor tissues from 24 individuals with cutaneous malignant melanoma of various stages. Our findings indicate that a tumor suppressor gene that plays a role in malignant melanoma is located on the long arm of chromosome 11, likely within a 51 cM region at 11q23. Its loss appears to be a late event in tumor progression and may serve as an indicator for a less favorable clinical outcome.

¹ Supported in part by Deutsche Forschungsgemeinschaft Grant He 2228/1-1 (R. A. H.) and the Verein zur Förderung dermatologischer Forschung e.V. (Mannheim, Germany). A. L. is a medical student trainee of the Howard Hughes Medical Institute.

² To whom requests for reprints should be addressed, at Ludwig Institute for Cancer Research, San Diego Branch, 9500 Gilman Drive, La Jolla, CA 92093-0660.

Received 3/27/95. Accepted 5/ 5/95.

This article has been cited by other articles:

				M. Stark and N. Hayward Genome-Wide Loss of Heterozygosity and Copy Number Analysis in Melanoma Using High-Density Single-Nucleotide Polymorphism Arrays Cancer Res., March 15, 2007; 67(6): 2632 - 2642. [Abstract] [Full Text] [PDF]

				T. Nakayama, B. Taback, R. Turner, D. L. Morton, and D. S. B. Hoon Molecular Clonality of In-Transit Melanoma Metastasis Am. J. Pathol., April 1, 2001; 158(4): 1371 - 1378. [Abstract] [Full Text] [PDF]

				W. W. Noll, D. R. Belloni, J. L. Rutter, C. A. Storm, A. R. Schned, L. Titus-Ernstoff, M. S. Ernstoff, and C. E. Brinckerhoff Loss of Heterozygosity on Chromosome 11q22-23 in Melanoma Is Associated with Retention of the Insertion Polymorphism in the Matrix Metalloproteinase-1 Promoter Am. J. Pathol., February 1, 2001; 158(2): 691 - 697. [Abstract] [Full Text] [PDF]

				R. A. Herbst, S. Mommert, U. Casper, E. K. Podewski, P. Kiehl, A. Kapp, and J. Weiss 11q23 Allelic Loss Is Associated with Regional Lymph Node Metastasis in Melanoma Clin. Cancer Res., August 1, 2000; 6(8): 3222 - 3227. [Abstract] [Full Text]

				M. C.Stern1, F. Benavides, E. A.Klingelberger, and C. J.Conti2 Allelotype analysis of chemically induced squamous cell carcinomas in F1 hybrids of two inbred mouse strains with different susceptibility to tumor progression Carcinogenesis, July 1, 2000; 21(7): 1297 - 1301. [Abstract] [Full Text] [PDF]

				Z. Gu, C. Flemington, T. Chittenden, and G. P. Zambetti ei24, a p53 Response Gene Involved in Growth Suppression and Apoptosis Mol. Cell. Biol., January 1, 2000; 20(1): 233 - 241. [Abstract] [Full Text]

				S. Knuutila, Y. Aalto, K. Autio, A.-M. Bjorkqvist, W.'e. El-Rifai, S. Hemmer, T. Huhta, E. Kettunen, S. Kiuru-Kuhlefelt, M. L. Larramendy, et al. DNA Copy Number Losses in Human Neoplasms Am. J. Pathol., September 1, 1999; 155(3): 683 - 694. [Abstract] [Full Text] [PDF]

				Y. Fujiwara, D. D. J. Chi, H. Wang, P. Keleman, D. L. Morton, R. Turner, and D. S. B. Hoon Plasma DNA Microsatellites as Tumor-specific Markers and Indicators of Tumor Progression in Melanoma Patients Cancer Res., April 1, 1999; 59(7): 1567 - 1571. [Abstract] [Full Text] [PDF]