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A Variant Glucocorticoid Receptor Messenger RNA Is Expressed in Multiple Myeloma Patients¹

Robert H. Lurie Cancer Center and Department of Medicine, Northwestern University, Chicago, Illinois 60611 [N. L. K., S. P., P. A. M., S. T. R.], and Hematology and Oncology, Mayo Clinic, Rochester, Minnesota 55905 [P. R. G.]

In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877–3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present in myeloma patients. We detected both the wild-type and variant GR transcripts in the patient isolate that was the source of our myeloma cell lines, in patients refractory to steroid treatment, and in healthy control subjects. Simultaneous amplification of wild-type and variant GR mRNAs indicates that the variant GR is more highly expressed in cells that are resistant to glucocorticoids. We hypothesize that the variant GR is a normal mRNA transcript that acts to modulate glucocorticoid responsiveness, and increased expression contributes to a resistant phenotype.

¹ This project was funded by the Eastern Cooperative Oncology Group, the American Cancer Society (Illinois Division), and National Cancer Institute Grant P30 CA60553-01 to the Robert H. Lurie Cancer Center.

² To whom requests for reprints should be addressed, at Northwest University Medical Center, R. H. Lurie Cancer Center, 8340 Olson Pavilion, 303 East Chicago Avenue, Chicago, IL 60611.

Received 3/20/95. Accepted 5/19/95.

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