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Application of a Tumor Suppressor (C-CAM1)-expressing Recombinant Adenovirus in Androgen-independent Human Prostate Cancer Therapy: A Preclinical Study¹

Departments of Urology [D. I. K., A. C. v. E., J. T. H.], Thoracic Surgery [W-W. Z.], Hematology [N. T. V.], and Molecular Pathology [S-H. L.], The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030

Recently, we demonstrated that an androgen-regulated cell adhesion molecule, C-CAM, acts as a tumor suppressor in prostate cancer development. In this study, we further explored the possibility of applying C-CAM as a potential agent for developing prostate cancer gene therapy using an adenoviral delivery system. We found that prostate cancer cells, in general, were sensitive to adenoviral infection. In vitro characterization indicated that C-CAM1 protein was detected only in C-CAM1 adenovirus-infected cells but not in antisense control virus-infected cells, and the levels of expression showed dose dependency. Because of the stability of the protein, C-CAM expression in viral-infected cells appeared to be a long-lasting event, indicating that C-CAM may be superior to many other known tumor suppressors that have a short protein half-life. Most importantly, the delivery of a single dose of C-CAM adenovirus was able to repress the growth of PC-3-induced tumors in nude mice for at least 3 weeks. Taken together, these data indicate that C-CAM is a potential candidate for human prostate cancer therapy.

¹ Supported in part by an American Cancer Society grant (to D. I. K.), NIH Grants GM 43189 (to S-H. L.), CA 59939 (to J-T. H.), and Core Grant CA 16672 (to the M. D. Anderson Cancer Center).

² To whom requests for reprints should be addressed, at Department of Urology, Box 026, M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030.

Received 1/30/95. Accepted 5/ 1/95.

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