This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Su, L.-K. Articles by Kinzler, K. W. Search for Related Content PubMed PubMed Citation Articles by Su, L.-K. Articles by Kinzler, K. W.

APC Binds to the Novel Protein EB1¹

The Johns Hopkins Oncology Center [L-K. S., K. W. K.] and The Howard Hughes Medical Institute and The Johns Hopkins Oncology Center [B. V.], Baltimore, Maryland 21231; Oncogene Science, Cambridge, Massachusetts 02142 [M. B., D. H.]; Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 [J. G., R. B.]; and National Center for Human Genome Research, Bethesda, Maryland 20892 [R. W., J. T.]

Mutations of the APC gene play a critical role in both sporadic and familial forms of colorectal cancer. The vast majority of these mutations result in the loss of the carboxyl terminus of the protein. To further elucidate the function of APC, we searched for cellular proteins that associate with its carboxyl terminus. One million human cDNA clones were screened with the use of the interaction trap two-hybrid system, and 67 clones were found to have a phenotype suggestive of an APC-interacting protein. Nucleotide sequence analysis revealed that 48 of these clones were derived from a single novel gene named EB1. The association of APC and EB1 proteins was confirmed with in vitro Binding assays. mAbs against EB1 were then produced and used to demonstrate the association of APC and EB1 in vivo. The EB1 gene was predicted to encode a 268-amino acid protein without significant homology to proteins with known function. However, searches of nucleotide databases did identify evidence for at least two related human genes and a yeast homologue. This conservation suggests an essential function for EB1 that might provide clues to the mechanism through which APC suppresses colonic neoplasia.

¹ This work was supported in part by grants from the Clayton Fund and NIH Grant CA-57345.

² Present address: Mitotix, Inc., 1 Kendall Square, Cambridge, MA 02139.

³ Recipient of an American Cancer Society Faculty Research Award.

⁴ American Cancer Society Research Professor and Investigator of The Howard Hughes Medical Institute.

⁵ To whom requests for reprints should be addressed, at The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231.

Received 5/12/95. Accepted 6/ 1/95.

This article has been cited by other articles:

				R. Ban, H. Matsuzaki, T. Akashi, G. Sakashita, H. Taniguchi, S.-Y. Park, H. Tanaka, K. Furukawa, and T. Urano Mitotic Regulation of the Stability of Microtubule Plus-end Tracking Protein EB3 by Ubiquitin Ligase SIAH-1 and Aurora Mitotic Kinases J. Biol. Chem., October 9, 2009; 284(41): 28367 - 28381. [Abstract] [Full Text] [PDF]

				T. Zhang, K. J. M. Zaal, J. Sheridan, A. Mehta, G. G. Gundersen, and E. Ralston Microtubule plus-end binding protein EB1 is necessary for muscle cell differentiation, elongation and fusion J. Cell Sci., May 1, 2009; 122(9): 1401 - 1409. [Abstract] [Full Text] [PDF]

				L. Guo, C.-M. K. Ho, Z. Kong, Y.-R. J. Lee, Q. Qian, and B. Liu Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome Ann. Bot., February 1, 2009; 103(3): 387 - 402. [Abstract] [Full Text] [PDF]

				C. Matsui, S. Kaieda, T. Ikegami, and Y. Mimori-Kiyosue Identification of a Link between the SAMP Repeats of Adenomatous Polyposis Coli Tumor Suppressor and the Src Homology 3 Domain of DDEF J. Biol. Chem., November 21, 2008; 283(47): 33006 - 33020. [Abstract] [Full Text] [PDF]

				Z. Li, K. Kroboth, I. P. Newton, and I. S. Nathke Novel self-association of the APC molecule affects APC clusters and cell migration J. Cell Sci., June 1, 2008; 121(11): 1916 - 1925. [Abstract] [Full Text] [PDF]

				L. Sun, J. Gao, X. Dong, M. Liu, D. Li, X. Shi, J.-T. Dong, X. Lu, C. Liu, and J. Zhou EB1 promotes Aurora-B kinase activity through blocking its inactivation by protein phosphatase 2A PNAS, May 20, 2008; 105(20): 7153 - 7158. [Abstract] [Full Text] [PDF]

				G. J. Buttrick, L. M.A. Beaumont, J. Leitch, C. Yau, J. R. Hughes, and J. G. Wakefield Akt regulates centrosome migration and spindle orientation in the early Drosophila melanogaster embryo J. Cell Biol., February 6, 2008; 180(3): 537 - 548. [Abstract] [Full Text] [PDF]

				S. R. Bisgrove, Y.-R. J. Lee, B. Liu, N. T. Peters, and D. L. Kropf The Microtubule Plus-End Binding Protein EB1 Functions in Root Responses to Touch and Gravity Signals in Arabidopsis PLANT CELL, February 1, 2008; 20(2): 396 - 410. [Abstract] [Full Text] [PDF]

				P. Goulimari, H. Knieling, U. Engel, and R. Grosse LARG and mDia1 Link G{alpha}12/13 to Cell Polarity and Microtubule Dynamics Mol. Biol. Cell, January 1, 2008; 19(1): 30 - 40. [Abstract] [Full Text] [PDF]

				J. M. Halbleib, A. M. Saaf, P. O. Brown, and W. J. Nelson Transcriptional Modulation of Genes Encoding Structural Characteristics of Differentiating Enterocytes During Development of a Polarized Epithelium In Vitro Mol. Biol. Cell, November 1, 2007; 18(11): 4261 - 4278. [Abstract] [Full Text] [PDF]

				K. Aoki and M. M. Taketo Adenomatous polyposis coli (APC): a multi-functional tumor suppressor gene J. Cell Sci., October 1, 2007; 120(19): 3327 - 3335. [Abstract] [Full Text] [PDF]

				C. M. Caldwell, R. A. Green, and K. B. Kaplan APC mutations lead to cytokinetic failures in vitro and tetraploid genotypes in Min mice J. Cell Biol., September 24, 2007; 178(7): 1109 - 1120. [Abstract] [Full Text] [PDF]

				J. Zhang, S. Ahmad, and Y. Mao BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment J. Cell Biol., August 27, 2007; 178(5): 773 - 784. [Abstract] [Full Text] [PDF]

				J. B. Moseley, F. Bartolini, K. Okada, Y. Wen, G. G. Gundersen, and B. L. Goode Regulated Binding of Adenomatous Polyposis Coli Protein to Actin J. Biol. Chem., April 27, 2007; 282(17): 12661 - 12668. [Abstract] [Full Text] [PDF]

				V. Neduva and R. B. Russell DILIMOT: discovery of linear motifs in proteins. Nucleic Acids Res., July 1, 2006; 34(Web Server issue): W350 - W355. [Abstract] [Full Text] [PDF]

				M. Sharma, L. Leung, M. Brocardo, J. Henderson, C. Flegg, and B. R. Henderson Membrane Localization of Adenomatous Polyposis Coli Protein at Cellular Protrusions: TARGETING SEQUENCES AND REGULATION BY beta-CATENIN J. Biol. Chem., June 23, 2006; 281(25): 17140 - 17149. [Abstract] [Full Text] [PDF]

				B. M. McCartney, M. H. Price, R. L. Webb, M. A. Hayden, L. M. Holot, M. Zhou, A. Bejsovec, and M. Peifer Testing hypotheses for the functions of APC family proteins using null and truncation alleles in Drosophila Development, June 15, 2006; 133(12): 2407 - 2418. [Abstract] [Full Text] [PDF]

				D. K. Moss, V. M. Betin, S. D. Malesinski, and J. D. Lane A novel role for microtubules in apoptotic chromatin dynamics and cellular fragmentation J. Cell Sci., June 1, 2006; 119(11): 2362 - 2374. [Abstract] [Full Text] [PDF]

				K. Kita, T. Wittmann, I. S. Nathke, and C. M. Waterman-Storer Adenomatous Polyposis Coli on Microtubule Plus Ends in Cell Extensions Can Promote Microtubule Net Growth with or without EB1 Mol. Biol. Cell, May 1, 2006; 17(5): 2331 - 2345. [Abstract] [Full Text] [PDF]

				K. Asakawa, K. Kume, M. Kanai, T. Goshima, K. Miyahara, S. Dhut, W. W. Tee, D. Hirata, and T. Toda The V260I Mutation in Fission Yeast {alpha}-Tubulin Atb2 Affects Microtubule Dynamics and EB1-Mal3 Localization and Activates the Bub1 Branch of the Spindle Checkpoint Mol. Biol. Cell, March 1, 2006; 17(3): 1421 - 1435. [Abstract] [Full Text] [PDF]

				X. Yan, R. Habedanck, and E. A. Nigg A Complex of Two Centrosomal Proteins, CAP350 and FOP, Cooperates with EB1 in Microtubule Anchoring Mol. Biol. Cell, February 1, 2006; 17(2): 634 - 644. [Abstract] [Full Text] [PDF]

				J. K. Moore, S. D'Silva, and R. K. Miller The CLIP-170 Homologue Bik1p Promotes the Phosphorylation and Asymmetric Localization of Kar9p Mol. Biol. Cell, January 1, 2006; 17(1): 178 - 191. [Abstract] [Full Text] [PDF]

				K. T. Vaughan TIP maker and TIP marker; EB1 as a master controller of microtubule plus ends J. Cell Biol., October 24, 2005; 171(2): 197 - 200. [Abstract] [Full Text] [PDF]

				R. A. Green, R. Wollman, and K. B. Kaplan APC and EB1 Function Together in Mitosis to Regulate Spindle Dynamics and Chromosome Alignment Mol. Biol. Cell, October 1, 2005; 16(10): 4609 - 4622. [Abstract] [Full Text] [PDF]

				N. S. Dzhindzhev, S. L. Rogers, R. D. Vale, and H. Ohkura Distinct mechanisms govern the localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus-ends J. Cell Sci., August 15, 2005; 118(16): 3781 - 3790. [Abstract] [Full Text] [PDF]

				K. C. Slep, S. L. Rogers, S. L. Elliott, H. Ohkura, P. A. Kolodziej, and R. D. Vale Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end J. Cell Biol., February 14, 2005; 168(4): 587 - 598. [Abstract] [Full Text] [PDF]

				K. Steigerwald, G. K. Behbehani, K. A. Combs, M. C. Barton, and J. Groden The APC Tumor Suppressor Promotes Transcription-Independent Apoptosis In vitro Mol. Cancer Res., February 1, 2005; 3(2): 78 - 89. [Abstract] [Full Text] [PDF]

				S. L. Elliott, C. F. Cullen, N. Wrobel, M. J. Kernan, and H. Ohkura EB1 Is Essential during Drosophila Development and Plays a Crucial Role in the Integrity of Chordotonal Mechanosensory Organs Mol. Biol. Cell, February 1, 2005; 16(2): 891 - 901. [Abstract] [Full Text] [PDF]

				Y. Mimori-Kiyosue, I. Grigoriev, G. Lansbergen, H. Sasaki, C. Matsui, F. Severin, N. Galjart, F. Grosveld, I. Vorobjev, S. Tsukita, et al. CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex J. Cell Biol., January 3, 2005; 168(1): 141 - 153. [Abstract] [Full Text] [PDF]

				A. Tighe, V. L. Johnson, and S. S. Taylor Truncating APC mutations have dominant effects on proliferation, spindle checkpoint control, survival and chromosome stability J. Cell Sci., December 15, 2004; 117(26): 6339 - 6353. [Abstract] [Full Text] [PDF]

				S. R. Bisgrove, W. E. Hable, and D. L. Kropf +TIPs and Microtubule Regulation. The Beginning of the Plus End in Plants Plant Physiology, December 1, 2004; 136(4): 3855 - 3863. [Full Text] [PDF]

				A. Kerres, C. Vietmeier-Decker, J. Ortiz, I. Karig, C. Beuter, J. Hegemann, J. Lechner, and U. Fleig The Fission Yeast Kinetochore Component Spc7 Associates with the EB1 Family Member Mal3 and Is Required for Kinetochore-Spindle Association Mol. Biol. Cell, December 1, 2004; 15(12): 5255 - 5267. [Abstract] [Full Text] [PDF]

				A. Brumby, J. Secombe, J. Horsfield, M. Coombe, N. Amin, D. Coates, R. Saint, and H. Richardson A Genetic Screen for Dominant Modifiers of a cyclin E Hypomorphic Mutation Identifies Novel Regulators of S-Phase Entry in Drosophila Genetics, September 1, 2004; 168(1): 227 - 251. [Abstract] [Full Text] [PDF]

				R. Fraschini, D. Bilotta, G. Lucchini, and S. Piatti Functional Characterization of Dma1 and Dma2, the Budding Yeast Homologues of Schizosaccharomyces pombe Dma1 and Human Chfr Mol. Biol. Cell, August 1, 2004; 15(8): 3796 - 3810. [Abstract] [Full Text] [PDF]

				M. K. Temburni, M. M. Rosenberg, N. Pathak, R. McConnell, and M. H. Jacob Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein J. Neurosci., July 28, 2004; 24(30): 6776 - 6784. [Abstract] [Full Text] [PDF]

				D. Dikovskaya, I. P. Newton, and I. S. Nathke The Adenomatous Polyposis Coli Protein Is Required for the Formation of Robust Spindles Formed in CSF Xenopus Extracts Mol. Biol. Cell, June 1, 2004; 15(6): 2978 - 2991. [Abstract] [Full Text] [PDF]

				M. L. Mayer, I. Pot, M. Chang, H. Xu, V. Aneliunas, T. Kwok, R. Newitt, R. Aebersold, C. Boone, G. W. Brown, et al. Identification of Protein Complexes Required for Efficient Sister Chromatid Cohesion Mol. Biol. Cell, April 1, 2004; 15(4): 1736 - 1745. [Abstract] [Full Text] [PDF]

				R. K. Louie, S. Bahmanyar, K. A. Siemers, V. Votin, P. Chang, T. Stearns, W. J. Nelson, and A. I. M. Barth Adenomatous polyposis coli and EB1 localize in close proximity of the mother centriole and EB1 is a functional component of centrosomes J. Cell Sci., March 1, 2004; 117(7): 1117 - 1128. [Abstract] [Full Text] [PDF]

				T. Sekiya, S. Adachi, K. Kohu, T. Yamada, O. Higuchi, Y. Furukawa, Y. Nakamura, T. Nakamura, K. Tashiro, S. Kuhara, et al. Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor-{beta} Signaling, as a Target of the {beta}-Catenin Pathway in Colorectal Tumor Cells J. Biol. Chem., February 20, 2004; 279(8): 6840 - 6846. [Abstract] [Full Text] [PDF]

				M. C. Faux, J. L. Ross, C. Meeker, T. Johns, H. Ji, R. J. Simpson, M. J. Layton, and A. W. Burgess Restoration of full-length adenomatous polyposis coli (APC) protein in a colon cancer cell line enhances cell adhesion J. Cell Sci., January 22, 2004; 117(3): 427 - 439. [Abstract] [Full Text] [PDF]

				M. Lee, S. Lee, A. D. Zadeh, and P. A. Kolodziej Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion Development, December 15, 2003; 130(24): 5989 - 5999. [Abstract] [Full Text] [PDF]

				W. Bu and L.-K. Su Characterization of Functional Domains of Human EB1 Family Proteins J. Biol. Chem., December 12, 2003; 278(50): 49721 - 49731. [Abstract] [Full Text] [PDF]

				R. A. Green and K. B. Kaplan Chromosome instability in colorectal tumor cells is associated with defects in microtubule plus-end attachments caused by a dominant mutation in APC J. Cell Biol., December 8, 2003; 163(5): 949 - 961. [Abstract] [Full Text] [PDF]

				I. Hayashi and M. Ikura Crystal Structure of the Amino-terminal Microtubule-binding Domain of End-binding Protein 1 (EB1) J. Biol. Chem., September 19, 2003; 278(38): 36430 - 36434. [Abstract] [Full Text] [PDF]

				L. A. Ligon, S. S. Shelly, M. Tokito, and E. L.F. Holzbaur The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization Mol. Biol. Cell, April 1, 2003; 14(4): 1405 - 1417. [Abstract] [Full Text] [PDF]

				N. S. Williams, R. B. Gaynor, S. Scoggin, U. Verma, T. Gokaslan, C. Simmang, J. Fleming, D. Tavana, E. Frenkel, and C. Becerra Identification and Validation of Genes Involved in the Pathogenesis of Colorectal Cancer Using cDNA Microarrays and RNA Interference Clin. Cancer Res., March 1, 2003; 9(3): 931 - 946. [Abstract] [Full Text] [PDF]

				J. S. Tirnauer, J. C. Canman, E.D. Salmon, and T. J. Mitchison EB1 Targets to Kinetochores with Attached, Polymerizing Microtubules Mol. Biol. Cell, December 1, 2002; 13(12): 4308 - 4316. [Abstract] [Full Text] [PDF]

				J. M. Askham, K. T. Vaughan, H. V. Goodson, and E. E. Morrison Evidence That an Interaction between EB1 and p150Glued Is Required for the Formation and Maintenance of a Radial Microtubule Array Anchored at the Centrosome Mol. Biol. Cell, October 1, 2002; 13(10): 3627 - 3645. [Abstract] [Full Text] [PDF]

				S. L. Rogers, G. C. Rogers, D. J. Sharp, and R. D. Vale Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle J. Cell Biol., September 3, 2002; 158(5): 873 - 884. [Abstract] [Full Text] [PDF]

				M. Rehberg and R. Graf Dictyostelium EB1 Is a Genuine Centrosomal Component Required for Proper Spindle Formation Mol. Biol. Cell, July 1, 2002; 13(7): 2301 - 2310. [Abstract] [Full Text] [PDF]

				M. M. Mogensen, J. B. Tucker, J. B. Mackie, A. R. Prescott, and I. S. Nathke The adenomatous polyposis coli protein unambiguously localizes to microtubule plus ends and is involved in establishing parallel arrays of microtubule bundles in highly polarized epithelial cells J. Cell Biol., June 10, 2002; 157(6): 1041 - 1048. [Abstract] [Full Text] [PDF]

				A. I. M. Barth, K. A. Siemers, and W. J. Nelson Dissecting interactions between EB1, microtubules and APC in cortical clusters at the plasma membrane J. Cell Sci., April 15, 2002; 115(8): 1583 - 1590. [Abstract] [Full Text] [PDF]

				M. A. Galea, A. Eleftheriou, and B. R. Henderson ARM Domain-dependent Nuclear Import of Adenomatous Polyposis Coli Protein Is Stimulated by the B56alpha Subunit of Protein Phosphatase 2A J. Biol. Chem., November 30, 2001; 276(49): 45833 - 45839. [Abstract] [Full Text] [PDF]

				Y. Mimori-Kiyosue and S. Tsukita Where is APC going? J. Cell Biol., September 17, 2001; 154(6): 1105 - 1110. [Abstract] [Full Text] [PDF]

				N. S. Fearnhead, M. P. Britton, and W. F. Bodmer The ABC of APC Hum. Mol. Genet., April 1, 2001; 10(7): 721 - 733. [Abstract] [Full Text] [PDF]

				R J Scott, C Meldrum, R Crooks, A D Spigelman, J Kirk, K Tucker, D Koorey, and t. H. F. C. Service Familial adenomatous polyposis: more evidence for disease diversity and genetic heterogeneity Gut, April 1, 2001; 48(4): 508 - 514. [Abstract] [Full Text] [PDF]

				K. STEIGERWALD, I. M SANTORO, J. J KORDICH, V. GISMONDI, C. TRZEPACZ, M. BADIALI, F GIANGASPERO, M G. BALKO, J. S GRAHAM, N. RATNER, et al. A distinct splice form of APC is highly expressed in neurones but not commonly mutated in neuroepithelial tumours J. Med. Genet., April 1, 2001; 38(4): 257 - 262. [Full Text]

				R. A. Wagenaar, H. C. Crawford, and L. M. Matrisian Stabilized {beta}-Catenin Immortalizes Colonic Epithelial Cells Cancer Res., March 1, 2001; 61(5): 2097 - 2104. [Abstract] [Full Text]

				A. Reinacher-Schick and B. M. Gumbiner Apical Membrane Localization of the Adenomatous Polyposis Coli Tumor Suppressor Protein and Subcellular Distribution of the {beta}-Catenin Destruction Complex in Polarized Epithelial Cells J. Cell Biol., February 5, 2001; 152(3): 491 - 502. [Abstract] [Full Text] [PDF]

				S. Schuyler and D Pellman Search, capture and signal: games microtubules and centrosomes play J. Cell Sci., January 1, 2001; 114(2): 247 - 255. [Abstract] [PDF]

				C.-R. Chen, J. Chen, and E. C. Chang A Conserved Interaction between Moe1 and Mal3 Is Important for Proper Spindle Formation in Schizosaccharomyces pombe Mol. Biol. Cell, December 1, 2000; 11(12): 4067 - 4077. [Abstract] [Full Text]

				R. K. Miller, S.-C. Cheng, and M. D. Rose Bim1p/Yeb1p Mediates the Kar9p-dependent Cortical Attachment of Cytoplasmic Microtubules Mol. Biol. Cell, September 1, 2000; 11(9): 2949 - 2959. [Abstract] [Full Text]

				Y. Kawasaki, T. Senda, T. Ishidate, R. Koyama, T. Morishita, Y. Iwayama, O. Higuchi, and T. Akiyama Asef, a Link Between the Tumor Suppressor APC and G-Protein Signaling Science, August 18, 2000; 289(5482): 1194 - 1197. [Abstract] [Full Text]

				J. S. Tirnauer and B. E. Bierer Eb1 Proteins Regulate Microtubule Dynamics, Cell Polarity, and Chromosome Stability J. Cell Biol., May 15, 2000; 149(4): 761 - 766. [Full Text] [PDF]

				K. H. Goss and J. Groden Biology of the Adenomatous Polyposis Coli Tumor Suppressor J. Clin. Oncol., May 9, 2000; 18(9): 1967 - 1979. [Abstract] [Full Text] [PDF]

				W. S. Korinek, M. J. Copeland, A. Chaudhuri, and J. Chant Molecular Linkage Underlying Microtubule Orientation Toward Cortical Sites in Yeast Science, March 24, 2000; 287(5461): 2257 - 2259. [Abstract] [Full Text]

				L. Lee, J. S. Tirnauer, J. Li, S. C. Schuyler, J. Y. Liu, and D. Pellman Positioning of the Mitotic Spindle by a Cortical-Microtubule Capture Mechanism Science, March 24, 2000; 287(5461): 2260 - 2262. [Abstract] [Full Text]

				K. Kawahara, T. Morishita, T. Nakamura, F. Hamada, K. Toyoshima, and T. Akiyama Down-regulation of beta -Catenin by the Colorectal Tumor Suppressor APC Requires Association with Axin and beta -Catenin J. Biol. Chem., March 17, 2000; 275(12): 8369 - 8374. [Abstract] [Full Text] [PDF]

				I.-M. Shih, J. Yu, T.-C. He, B. Vogelstein, and K. W. Kinzler The {beta}-Catenin Binding Domain of Adenomatous Polyposis Coli Is Sufficient for Tumor Suppression Cancer Res., March 1, 2000; 60(6): 1671 - 1676. [Abstract] [Full Text]

				Y. Mimori-Kiyosue, N. Shiina, and S. Tsukita Adenomatous Polyposis Coli (APC) Protein Moves along Microtubules and Concentrates at Their Growing Ends in Epithelial Cells J. Cell Biol., February 7, 2000; 148(3): 505 - 518. [Abstract] [Full Text] [PDF]

				H. Nakagawa, K. Koyama, Y. Murata, M. Morito, T. Akiyama, and Y. Nakamura APCL, a Central Nervous System-specific Homologue of Adenomatous Polyposis Coli Tumor Suppressor, Binds to p53-binding Protein 2 and Translocates it to the Perinucleus Cancer Res., January 1, 2000; 60(1): 101 - 105. [Abstract] [Full Text]

				T. P. Moynihan, H. C. Ardley, U. Nuber, S. A. Rose, P. F. Jones, A. F. Markham, M. Scheffner, and P. A. Robinson The Ubiquitin-conjugating Enzymes UbcH7 and UbcH8 Interact with RING Finger/IBR Motif-containing Domains of HHARI and H7-AP1 J. Biol. Chem., October 22, 1999; 274(43): 30963 - 30968. [Abstract] [Full Text] [PDF]

				B. M. McCartney, H. A. Dierick, C. Kirkpatrick, M. M. Moline, A. Baas, M. Peifer, and A. Bejsovec Drosophila Apc2 Is a Cytoskeletally-Associated Protein That Regulates Wingless Signaling in the Embryonic Epidermis J. Cell Biol., September 20, 1999; 146(6): 1303 - 1318. [Abstract] [Full Text] [PDF]

				T. Senda, A. Matsumine, H. Yanai, and T. Akiyama Localization of MCC (Mutated in Colorectal Cancer) in Various Tissues of Mice and Its Involvement in Cell Differentiation J. Histochem. Cytochem., September 1, 1999; 47(9): 1149 - 1158. [Abstract] [Full Text]

				K. Caca, F. T. Kolligs, X. Ji, M. Hayes, J.-m. Qian, A. Yahanda, D. L. Rimm, J. Costa, and E. R. Fearon {beta}- and {{gamma}}-Catenin Mutations, but not E-Cadherin Inactivation, Underlie T-Cell Factor/Lymphoid Enhancer Factor Transcriptional Deregulation in Gastric and Pancreatic Cancer Cell Growth Differ., June 1, 1999; 10(6): 369 - 376. [Abstract] [Full Text]

				J. S. Tirnauer, E. O'Toole, L. Berrueta, B. E. Bierer, and D. Pellman Yeast Bim1p Promotes the G1-specific Dynamics of Microtubules J. Cell Biol., May 31, 1999; 145(5): 993 - 1007. [Abstract] [Full Text] [PDF]

				R. Smits, M. F. Kielman, C. Breukel, C. Zurcher, K. Neufeld, S. Jagmohan-Changur, N. Hofland, J. van Dijk, R. White, W. Edelmann, et al. Apc1638T: a mouse model delineating critical domains of the adenomatous polyposis coli protein involved in tumorigenesis and development Genes & Dev., May 15, 1999; 13(10): 1309 - 1321. [Abstract] [Full Text]

				N. N. Mahmoud, R. T. Bilinski, M. R. Churchill, W. Edelmann, R. Kucherlapati, and M. M. Bertagnolli Genotype-Phenotype Correlation in Murine Apc Mutation: Differences in Enterocyte Migration and Response to Sulindac Cancer Res., January 1, 1999; 59(2): 353 - 359. [Abstract] [Full Text] [PDF]

				N. Ratner, G. S. Bloom, and S. T. Brady A Role for Cyclin-Dependent Kinase(s) in the Modulation of Fast Anterograde Axonal Transport: Effects Defined by Olomoucine and the APC Tumor Suppressor Protein J. Neurosci., October 1, 1998; 18(19): 7717 - 7726. [Abstract] [Full Text] [PDF]

				L. Berrueta, S.-K. Kraeft, J. S. Tirnauer, S. C. Schuyler, L. B. Chen, D. E. Hill, D. Pellman, and B. E. Bierer The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules PNAS, September 1, 1998; 95(18): 10596 - 10601. [Abstract] [Full Text] [PDF]

				K. Schwartz, K. Richards, and D. Botstein BIM1 Encodes a Microtubule-binding Protein in Yeast Mol. Biol. Cell, December 1, 1997; 8(12): 2677 - 2691. [Abstract] [Full Text]

				J. D. Beinhauer, I. M. Hagan, J. H. Hegemann, and U. Fleig Mal3, the Fission Yeast Homologue of the Human APC-interacting Protein EB-1 Is Required for Microtubule Integrity and the Maintenance of Cell Form J. Cell Biol., November 3, 1997; 139(3): 717 - 728. [Abstract] [Full Text] [PDF]

				C. Trzepacz, A. M. Lowy, J. J. Kordich, and J. Groden Phosphorylation of the Tumor Suppressor Adenomatous Polyposis Coli (APC) by the Cyclin-dependent Kinase p34cdc2 J. Biol. Chem., August 29, 1997; 272(35): 21681 - 21684. [Abstract] [Full Text] [PDF]

				K. Vleminckx, E. Wong, K. Guger, B. Rubinfeld, P. Polakis, and B. M. Gumbiner Adenomatous Polyposis Coli Tumor Suppressor Protein Has Signaling Activity in Xenopus laevis Embryos Resulting in the Induction of an Ectopic Dorsoanterior Axis J. Cell Biol., January 27, 1997; 136(2): 411 - 420. [Abstract] [Full Text] [PDF]

				S. Hayashi, B. Rubinfeld, B. Souza, P. Polakis, E. Wieschaus, and A. J. Levine A Drosophila homolog of the tumor suppressor gene adenomatous polyposis coli down-regulates beta -catenin but its zygotic expression is not essential for the regulation of Armadillo PNAS, January 7, 1997; 94(1): 242 - 247. [Abstract] [Full Text] [PDF]