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Frequent Novel DNA Copy Number Increase in Squamous Cell Head and Neck Tumors¹

Department of Radiation Oncology [P. M. B., N. A. L., K. K. F., J. W. G., M. F. C.] and Division of Molecular Cytometry in the Department of Laboratory Medicine [N. A. L., J. W. G.], and Department of Otolaryngology [M. J. K., M. I. S.], University of California, San Francisco, California 94143

We have undertaken a study of DNA copy number changes in head and neck squamous cell carcinomas to identify novel DNA copy number changes and to determine the significance of previous findlings of cytogenetic alterations in cultured cells. Comparative genomic hybridization was performed on geomic DNA extracted from ten tumors. A novel copy number gain on chromosome 3q26–27 and a loss of chromosome 3p were found at high frequency (50% of tumors). Many other novel chromosomal copy number changes were identified but occurred at a lower frequency. In addition, our data confirm that DNA copy number changes that frequently occur in cultured cells, such as loss of chromosome 3p, also occur in tumors. Frequently altered loci may encode oncogenes or tumor suppressor genes involved in head and neck squamous cell carcinoma tumorigenesis.

¹ This study was supported in part by both the United States Department of Energy (under contract DE-AC-03-76SF00098) and Imagenetics.

² To whom requests for reprints should be addressed, at Department of Radiation Oncology, University of California, 1855 Folsom Street, MCB200, Box 0806, San Francisco, CA 94103.

Received 12/16/94. Accepted 5/16/95.

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