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Heterogeneity of Deletions Involving RB-1 and the D13S25 Locus in B-Cell Chronic Lymphocytic Leukemia Revealed by Fluorescence in Situ Hybridization¹

Medizinische Klinik und Poliklinik V, Universität Heidelberg, Hospitalstrasse 3, 69115 Heidelberg [S. S., E. L., G. W., M. S., K. F., M. B., H. D.], and Abteilung Organisation Komplexer Genome, Deutsches Krebsforschungszentrum, 69120 Heidelberg [S. O., P. L.], Germany

Recently, the D13S25 locus, which is in close proximity to the retinoblastoma gene (RB-1) on chromosome band 13q14, was discussed to play a role in the pathogenesis of B-CLL. In the present study, we isolated two overlapping genomic DNA clones (termed c13S25) containing the D13S25 DNA segment and used them as probes to analyze 85 B-CLL cases by fluorescence in situ hybridization; of the 55 cases with two RB-1 copies, 13 exhibited hemizygous (n = 7) or homozygous (n = 6) deletion of D13S25. Of 29 cases with hemizygous deletion of RB-1, all but two also showed loss of D13S25 (hemizygous, n = 25; homozygous, n = 2). One case had a homozygous deletion of both loci. We conclude that deletion of D13S25 occurs in a substantial number of B-CLL without deletion of RB-1. However, in some cases there is deletion of RB-1 without loss of D13S25, suggesting that D13S25 is not the locus of the putative tumor suppressor gene. According to our data, such a gene is most likely located within the genomic region between D13S25 and RB-1.

¹ This work was supported by grants from the Tumorzentrum Heidelberg/Mannheim (Forschungsschwerpunkt I/I.1) and the European Community (GENE-CT 930055).

² To whom requests for reprints should be addressed.

Received 5/ 9/95. Accepted 6/29/95.

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