| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Oncology, Roche Research Center, Hoffmann-LaRoche Inc., Nutley, New Jersey 07110
The p53 tumor suppressor is a transcription factor frequently mutated in human malignancies. Tumor-derived p53 missense mutants are defective in sequence-specific DNA binding and fall to activate p53 target genes. mAb PAb421 was shown previously to restore DNA binding to selected p53 mutants in vitro. Here we show that mAb PAb421 when microinjected into human SW480 colorectal carcinoma cells restores the transcription activation function to the resident mutant p53 (arg to his 273, pro to ser 309). Codon 273 is the second most frequent p53 missense mutant found in human tumors. Our results lend support to the concept of restoring wild-type function to mutant p53 as a strategy for cancer therapy.
1 To whom requests for reprints should be addressed.
Received 4/18/95. Accepted 7/ 6/95.
This article has been cited by other articles:
|
|
 |
|
  E. Meyer, J.-Y. Vollmer, R. Bovey, and I. Stamenkovic Matrix Metalloproteinases 9 and 10 Inhibit Protein Kinase C-Potentiated, p53-Mediated Apoptosis Cancer Res., May 15, 2005; 65(10): 4261 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Demma, S. Wong, E. Maxwell, and B. Dasmahapatra CP-31398 Restores DNA-binding Activity to Mutant p53 in Vitro but Does Not Affect p53 Homologs p63 and p73 J. Biol. Chem., October 29, 2004; 279(44): 45887 - 45896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sharma, A. Pradeep, L. Wong, A. Rana, and B. Rana Peroxisome Proliferator-activated Receptor {gamma} Activation Can Regulate {beta}-Catenin Levels via a Proteasome-mediated and Adenomatous Polyposis Coli-independent Pathway J. Biol. Chem., August 20, 2004; 279(34): 35583 - 35594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Xiao, C. Ghosn, C. Hinchman, C. Forbes, J. Wang, N. Snider, A. Cordrey, Y. Zhao, and R. A. S. Chandraratna Adenomatous Polyposis Coli (APC)-independent Regulation of {beta}-Catenin Degradation via a Retinoid X Receptor-mediated Pathway J. Biol. Chem., August 8, 2003; 278(32): 29954 - 29962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kasibhatla and B. Tseng Why Target Apoptosis in Cancer Treatment? Mol. Cancer Ther., June 1, 2003; 2(6): 573 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Shagin, D. V. Rebrikov, V. B. Kozhemyako, I. M. Altshuler, A. S. Shcheglov, P. A. Zhulidov, E. A. Bogdanova, D. B. Staroverov, V. A. Rasskazov, and S. Lukyanov A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas Genome Res., December 1, 2002; 12(12): 1935 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. C. S. Wong and M. Pignatelli {beta}-Catenin--A Linchpin in Colorectal Carcinogenesis? Am. J. Pathol., February 1, 2002; 160(2): 389 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sadot, B. Geiger, M. Oren, and A. Ben-Ze'ev Down-Regulation of {beta}-Catenin by Activated p53 Mol. Cell. Biol., October 15, 2001; 21(20): 6768 - 6781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Pokrovskaja, K. Mattsson, E. Kashuba, G. Klein, and L. Szekely Proteasome inhibitor induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5 J. Gen. Virol., February 1, 2001; 82(2): 345 - 358. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sigal and V. Rotter Oncogenic Mutations of the p53 Tumor Suppressor: The Demons of the Guardian of the Genome Cancer Res., December 1, 2000; 60(24): 6788 - 6793. [Abstract] [Full Text]
|
|
|
|
|
|
S. Cambier, D.-z. Mu, D. O’Connell, K. Boylen, W. Travis, W.-h. Liu, V. C. Broaddus, and S. L. Nishimura A Role for the Integrin {{alpha}}v{beta}8 in the Negative Regulation of Epithelial Cell Growth Cancer Res., December 1, 2000; 60(24): 7084 - 7093. [Abstract] [Full Text]
|
|
|
|
|
|
H.-y. Tang, K. Zhao, J. F. Pizzolato, M. Fonarev, J. C. Langer, and J. J. Manfredi Constitutive Expression of the Cyclin-dependent Kinase Inhibitor p21 Is Transcriptionally Regulated by the Tumor Suppressor Protein p53 J. Biol. Chem., October 30, 1998; 273(44): 29156 - 29163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Perz and J. G. Kuhn Review : p53 in the pathogenesis, diagnosis, and treatment of cancer Journal of Oncology Pharmacy Practice, June 1, 1998; 4(2): 75 - 102. [Abstract] [PDF]
|
|
|
|
|
|
L. Whitesell, P. D. Sutphin, E. J. Pulcini, J. D. Martinez, and P. H. Cook The Physical Association of Multiple Molecular Chaperone Proteins with Mutant p53 Is Altered by Geldanamycin, an hsp90-Binding Agent Mol. Cell. Biol., March 1, 1998; 18(3): 1517 - 1524. [Abstract] [Full Text]
|
|
|
|
|
|
V. Gire and D. Wynford-Thomas Reinitiation of DNA Synthesis and Cell Division in Senescent Human Fibroblasts by Microinjection of Anti-p53 Antibodies Mol. Cell. Biol., March 1, 1998; 18(3): 1611 - 1621. [Abstract] [Full Text]
|
|
|
|
 |
|
 U.M. Moll and L.M. Schramm p53--An Acrobat in Tumorigenesis Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(1): 23 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hansen, C. A. Midgley, D. P. Lane, B. C. Freeman, R. I. Morimoto, and T. R. Hupp Modification of Two Distinct COOH-terminal Domains Is Required for Murine p53 Activation by Bacterial Hsp70 J. Biol. Chem., November 29, 1996; 271(48): 30922 - 30928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Friedlander, Y. Legros, T. Soussi, and C. Prives Regulation of Mutant p53 Temperature-sensitive DNA Binding J. Biol. Chem., October 11, 1996; 271(41): 25468 - 25478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L J Ko and C Prives p53: puzzle and paradigm. Genes & Dev., May 1, 1996; 10(9): 1054 - 1072. [PDF]
|
|
|
|
|
|
C. Cain, S. Miller, J. Ahn, and C. Prives The N Terminus of p53 Regulates Its Dissociation from DNA J. Biol. Chem., December 15, 2000; 275(51): 39944 - 39953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Blaydes, M. G. Luciani, S. Pospisilova, H. M.-L. Ball, B. Vojtesek, and T. R. Hupp Stoichiometric Phosphorylation of Human p53 at Ser315 Stimulates p53-dependent Transcription J. Biol. Chem., February 9, 2001; 276(7): 4699 - 4708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Zelphati, Y. Wang, S. Kitada, J. C. Reed, P. L. Felgner, and J. Corbeil Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System J. Biol. Chem., September 7, 2001; 276(37): 35103 - 35110. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
