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Aberrant Expression of the Growth Factor Wnt-5A in Human Malignancy¹

Department of Pathology, Anatomy, and Cell Biology and the Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

The Wnt-5A gene codes for a secreted cysteine-rich growth factor that mediates cell to cell signaling via a paracrine mechanism during development and ontogeny. We have recently determined the genomic organization and chromosomal mapping of the human Wnt-5A, and observed distinct patterns of expression in developing human embryos. In this report, we have performed a detailed expression analysis of 100 adult human tissues and tumors and 10 human cell lines. Our data show a widespread expression of Wnt-5A in adult tissues and cells, and aberrant mRNA levels in lung, breast, and prostate carcinomas and in melanomas. The up-regulation of Wnt-5A in human malignancy was not due to either gene rearrangement or amplification. These findings document an abnormal expression of this growth factor in malignancy and implicate Wnt-5A in the genesis of human cancer.

¹ This work was supported by NIH Grants RO1 CA39481 and RO1 CA47282 (R. V. I.). R.V.I. is a recipient of a Faculty Research Award (FRA-376) from the American Cancer Society.

² To whom requests for reprints should be addressed, at Department of Pathology, Anatomy, and Cell Biology, Room 249, Jefferson Alumni Hall, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107.

Received 4/20/95. Accepted 6/28/95.

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