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Human Breast Carcinoma Cells Transfected with the Gene Encoding a c-myc Promoter-binding Protein (MBP-1) Inhibits Tumors in Nude Mice¹

Department of Internal Medicine [R. B. R., R. S.], Institute for Molecular Virology [R. B. R.] and Pediatric Research Institute [E. S., M. H.], Saint Louis University School of Medicine, St. Louis, Missouri 63110

We have identified previously a gene from a human cervical carcinoma cell (HeLa) cDNA expression library that encodes a M_r 37,000 c-myc promoter-binding protein (MBP-1), which binds to the TATA box sequences of c-myc P2 promoter and exerts a negative regulatory function by down-regulating c-myc expression. In normal human tissues, this cloned gene showed variable expression. In this study, we have demonstrated that introduction of the MBP-1 gene into human breast carcinoma cells reduced their ability to invade through a basement membrane matrix in vitro but did not alter their growth rate. Human breast carcinoma transfected with MBP-1 cells showed a loss of anchorage-independent growth and also suppressed tumor formation in athymic nude mice. These results suggest the possible involvement of MBP-1 as a tumor suppressor gene in human breast carcinoma cells.

¹ This work was supported by National Cancer Institute Grant (R29 CA52799) and by an internal grant from the Department of Medicine, Saint Louis University (R. B. R.).

² To whom requests for reprints should be addressed, at Institute for Molecular Virology, Saint Louis University, 3681 Park Avenue, St. Louis, MO 63110.

Received 6/13/95. Accepted 7/24/95.

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